Liver bioengineering: Current status and future perspectives

Figure 1 Gross and microscopic anatomy of acellular ferret livers. Upper row: The liver on the left is almost entirely decellularized, however it remains a segment still cellular (interrupted line); on the left, instead, the liver is fully acellular as expression of successful decellularization; Middle row: Scanning electronic microscopic ruling out the presence of any cell remnant and showing the triad completely acellular (arrow); Lower row: Hematoxylin and eosin confirms the lack of cellular element within the remaining liver extracellular matrix (arrow).

Figure 2 Movat-Pentachrome staining of acellular liver sections shows yellow staining for collagen and dark staining for elastin surrounding the vascular structures.