In vitro effect of pantoprazole on lower esophageal sphincter tone in rats

Figure 1 Outline of the experimental procedure. A: The tissues were allowed to stabilize for 60 min in Krebs-containing organ baths. Following that period, their contractile response to 10^-6 mol/L carbachol was obtained. Pantoprazole was treated with 0.1 mol/L HCl and the pH of the drug solution was adjusted to 4.0. Different concentrations of pantoprazole were added directly to the tissue bath to generate cumulative concentrations of 5 × 10^-6 mol/L, 5 × 10^-5 mol/L and 1.5 × 10^-4 mol/L. The relaxations were quantified by integrating area under the curve for each concentration; B: For the control experiments, acidified distilled water was added at the same time points. LES: Lower esophageal sphincter.

Figure 2 Relaxation vs Dosage. 5 × 10^-5 mol/L and 1.5 × 10^-4 mol/L pantoprazole sodium induced significant relaxation in lower esophageal sphincter preparations in vitro (^aP < 0.05 and ^bP < 0.001). Each bar represents percent relaxation ± SEM for both control and experiment groups. Numbers in parentheses indicate the number of preparations used from different animals.