Brief Article
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World J Gastroenterol. Dec 14, 2011; 17(46): 5105-5109
Published online Dec 14, 2011. doi: 10.3748/wjg.v17.i46.5105
In vitro effect of pantoprazole on lower esophageal sphincter tone in rats
Mustafa Duman, Mahmut Özer, Enver Reyhan, Yeliz Demirci, Ali E Atıcı, Tahsin Dalgıç, Erdal B Bostancı, Ece Genç
Mustafa Duman, Enver Reyhan, Ali E Atıcı, Tahsin Dalgıç, Erdal B Bostancı, Department of Gastrointestinal Surgery, Kartal Kosuyolu Education and Research Hospital, Cevizli, Kartal, 34846 Istanbul, Turkey
Mahmut Özer, Yeliz Demirci, Ece Genç, Department of Pharmacology, Medical School, Yeditepe University, Atasehir, 34755 Istanbul, Turkey
Author contributions: Duman M, Özer M and Demirci Y performed the majority of the experiments; Reyhan E, Atıcı AE and Dalgıç T conducted the statistical analysis and interpreted the results; Bostancı EB provided the necessary chemicals and contributed to the design of the experiment; Genç E contributed to the design of the study and writing of the manuscript.
Correspondence to: Ece Genç, Professor, Department of Pharmacology, Medical School, Yeditepe University, Kayışdağı, Ataşehir, 34755 Istanbul, Turkey.
Telephone: +90-216-5780528 Fax: +90-216-5780575
Received: February 2, 2011
Revised: March 28, 2011
Accepted: April 18, 2011
Published online: December 14, 2011

AIM: To investigate the in vitro effects of pantoprazole on rat lower esophageal sphincter (LES) tone.

METHODS: Rats weighing 250-300 g, provided by the Yeditepe University Experimental Research Center (YÜDETAM), were used throughout the study. They were anesthetized before decapitation. LES tissues whose mucosal lining were removed were placed in a standard 30-mL organ bath with a modified Krebs solution and continuously aerated with 95% oxygen-5% carbon dioxide gas mixture and kept at room temperature. The tissues were allowed to stabilize for 60 min. Subsequently, the contractile response to 10-6 mol/L carbachol was obtained. Different concentrations of freshly prepared pantoprazole were added directly to the tissue bath to generate cumulative concentrations of 5 × 10-6 mol/L, 5 × 10-5 mol/L, and 1.5 × 10-4 mol/L. Activities were recorded on an online computer via a 4-channel transducer data acquisition system using the software BSL PRO v 3.7, which also analyzed the data.

RESULTS: Pantoprazole at 5 × 10-6 mol/L caused a small, but statistically insignificant, relaxation in the carbachol-contracted LES (2.23% vs 3.95%). The 5 × 10-5 mol/L concentration, however, caused a significant relaxation of 10.47% compared with the control. 1.5 × 10-4 mol/L concentration of pantoprazol caused a 19.89% relaxation in the carbachol contracted LES (P < 0.001).

CONCLUSION: This is the first study to demonstrate that pantoprazole has a relaxing effect in isolated LESs. These results might have significant clinical implications for the subset of patients using proton pump inhibitors who do not receive full symptomatic alleviation from gastroesophageal reflux disease.

Keywords: Pantoprazole, Lower esophageal sphincter, Gastroesophageal reflux disease