Computational prediction and experimental validation of novel markers for detection of STEC O157:H7

Figure 1 Distance distribution histogram for the barcode k-mer compositions of each gene compared with the average k-mer compositions of the whole genome. The x-axis is the k-mer genomic barcode distance, and the y-axis is the frequency. The bar indicates the frequency of different ranges of k-mer distance throughout the whole genome. The curve is the fitting curve to the histogram.

Figure 2 Diagnostic specificity of O157:H7 by one representative gene (eaeA) and three of our new markers. Lane M: E-Gel^® 1 Kb Plus DNA Ladder (Invitrogen); Lane C1: E. coli W3110; Lane C2: E. coli ATCC25922; Lane C3: S. aureus ATCC25923; Lane C4: P. aeruginosa ATCC27853; Lane C5: K. pneumonia ATCC700603; Lanes 1-18: O157:H7 bacterial isolates.