Omeprazole decreases magnesium transport across Caco-2 monolayers

Figure 1 Mg_A→B transport across Caco-2 monolayers. For Mg_A→B transport of 14 d monolayers, A: Control; B: 200 ng/mL omeprazole-treated; C: 400 ng/mL omeprazole-treated; D: 600 ng/mL omeprazole-treated; E: 800 ng/mL omeprazole-treated; and F: 1000 ng/mL omeprazole-treated monolayers. For Mg_A→B transport of 21 d monolayers, G: control; H: 200 ng/mL omeprazole-treated; I: 400 ng/mL omeprazole-treated; J: 600 ng/mL omeprazole-treated; K: 800 ng/mL omeprazole-treated and L: 1000 ng/mL omeprazole-treated monolayers. Light solid lines represent the saturable component. Dashed lines represent the non-saturable component. ^aP < 0.05, ^bP < 0.01, ^dP < 0.001 vs the age-matched control group, ^cP < 0.05, ^fP < 0.01, ^hP < 0.001 vs the concentration-matched 14 d omeprazole-treated groups. For each data point, n = 9.

Figure 2 Apical to basolateral P_Mg. A: P_Mg of 14 d control and various dose omeprazole-treated monolayers; B: 21 d control and various dose omeprazole-treated monolayers. ^aP < 0.05 vs the age-matched control group, ^cP < 0.05 vs the concentration-matched 14 d omeprazole-treated group. For each data point, n = 9.

Figure 3 Mg_B→A transport and basolateral to apical P_Mg. A: Mg_B→A transport; B: Basolateral to apical P_Mg of 14 d control and various dose omeprazole-treated monolayers. ^bP < 0.01 vs the control group. For each data point, n = 6.

Figure 4 Paracellular charge selectivity and selective permeability profile. A: P_Na/P_Cl; B: P_Na; C: P_Cl of 14 and 21 d control and various dose omeprazole-treated monolayers; D: Absolute alkaline metal ions (Li⁺, Na⁺, K⁺, Rb⁺, and Cs⁺) permeability; E: Trans-epithelium resistance (TER) of 14 d control and 600 ng/mL omeprazole-treated monolayers. ^aP < 0.05, ^bP < 0.01 vs the age matched control group. For each data point, n = 6.

Figure 5 Mechanism of Mg_A→B transport. A: Mg_A→B transport 14 d control and 600 ng/mL omeprazole-treated monolayers with or without I_SC; B and C: 20 μmol/L ruthenium red (RR); D: 100 μmol/L phlorizin or 100 μmol/L phloretin. For each data point, n = 9 in A; n = 6 in B and C. E, Arrhenius plot of 14 d control and 600 ng/mL omeprazole-treated monolayers. ^bP < 0.01 vs the control group. For each data point, n = 6.