Effect of 5-Aza-2’-deoxycytidine on immune-associated proteins in exosomes from hepatoma

Figure 1 Immunoelectron microscopy showing colloidal gold HSP70 labeled HepG2 exosomes in control group (A) and in experiment group (B), colloidal gold HLA-I labeled HepG2 exosomes in control group (C) and experiment group (D), colloidal gold NY-ESO-1 labeled HepG2 exosomes in control group (E) and experiment group (F), colloidal gold HSP70 labeled Hep3B exosomes in control group (G) and experiment group (H), colloidal gold HLA-I labeled Hep3B exosomes in control group (I) and experiment group (J), colloidal gold NY-ESO-1 labeled Hep3B exosomes in control group (K) and experiment group (L) (×140 000).

Figure 2 Immune molecules Western blotting results (A), semi-quantities results of Western blotting (B). 1: HepG2 control; 2: HepG2 experiment; 3: Hep3B control; 4: Hep3B experiment.

Figure 3 RT-PCR results of p53 gene expression (A), semi-quantities results of RT-PCR (B). M: Marker; C: Control group; E: Experiment group.