Suppression of gastric cancer growth by baculovirus vector-mediated transfer of normal epithelial cell specific-1 gene

Figure 1 Microscopy (A) and fluorescence (B) photos of SGC-7901 cells infected with recombinant baculoviruses (Bac-GFP) (× 40).

Figure 2 Western blot analysis showing NES1 protein expression in SGC-7901 cells. Lane 1: SGC-7901 cells + PBS; lane 2: SGC-7901 cells + Bac-GFP; lane 3: SGC-7901 cells + Bac-NES1.

Figure 3 Effect of infection with Bac-NES1 on growth of GC in vitro.

Figure 4 In vivo transgene expression in xenografted GC mediated by baculovirus carrying GFP (× 200) in Bac-NES1 treated group (A), Bac-GFP treated group (B), and PBS- treated group (C).

Figure 5 Inhibition of GC growth in nude mice. The mice were assigned to three groups (10 animals in each group). Growth of SGC-7901 xenografted tumor was measured at indicated time points. Relative tumor volume of the treatment groups was significantly different from that of the control group on day 27 of treatment (P < 0.01).

Figure 6 Immunohistochemical staining for tumors treated with Bac-NES1 (A), Bac-GFP (B), PBS (C), and the expression of NES1 protein in Bac-NES1-treated tumors (D). Data are presented as mean ± SD.