Gastric Cancer
Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Oct 14, 2008; 14(38): 5810-5815
Published online Oct 14, 2008. doi: 10.3748/wjg.14.5810
Suppression of gastric cancer growth by baculovirus vector-mediated transfer of normal epithelial cell specific-1 gene
Wei Huang, Xiang-Long Tian, Yun-Lin Wu, Jie Zhong, Li-Fen Yu, Sheng-Ping Hu, Biao Li
Wei Huang, Sheng-Ping Hu, Biao Li, Department of Nuclear Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
Wei Huang, Xiang-Long Tian, Yun-Lin Wu, Jie Zhong, Li-Fen Yu, Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
Author contributions: Li B, Wu YL, Zhong J and Yu LF designed the research; Huang W, Tian XL and Hu SP performed the research; Huang W and Tian XL analyzed the data; Huang W and Li B wrote the paper.
Supported by The Doctoral Fund from the Ministry of Education of China, No. BXJ0710
Correspondence to: Biao Li, Department of Nuclear Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China. lb10363@rjh.com.cn
Telephone: +86-21-64370045-665558 Fax: +86-21-64333548
Received: April 9, 2008
Revised: September 2, 2008
Accepted: September 9, 2008
Published online: October 14, 2008
Abstract

AIM: To study the inhibitory effect of baculovirus-mediated normal epithelial cell specific-1 (NES1) gene therapy on gastric cancer (GC) in vitro and in vivo.

METHODS: We first constructed recombinant baculovirus vectors and then transfected them into gastric cancer cells (SGC-7901). Efficiency of the baculovirus for gene transfer into SGC-7901 cells and cell growth curves were detected by fluorescence microscopy, Western blot and 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in vitro, respectively. The therapeutic effect of this gene therapy on GC was confirmed in xenografted nude mice. Tumor growth was determined by tumor volume, and expression of NES1 in tumor was analyzed by immunohistochemistry.

RESULTS: Baculovirus vectors were successfully transfected into SGC-7901 cells. SGC-7901 cells transfected with the NES1 gene inhibited cell growth. In the Bac-NES1 treated group, tumor growth was significantly reduced with a high level of NES1 expression

CONCLUSION: Baculovirus-mediated NES1 gene can be used in gene therapy for GC.

Keywords: Gastric cancer; Normal epithelial cell specific-1 gene; Baculovirus; Gene therapy