Diversity of Helicobacter pylori isolates in expression of antigens and induction of antibodies

Figure 1 H pylori isolated from a patients’ biopsy (Gram-staining, × 1000).

Figure 2 Expression and purification of target recombinant proteins. M: Marker (BioColor); Lane 1: Blank expression vector; Lanes 2-8 and 9-15: rUreB, rHpaA, rVacA, rCagA1, rNapA, rFlaA and rFlaB, respectively.

Figure 3 Detection results of target recombinant proteins by Western blot assay. M: Marker (BioColor); Lanes 1a-7a: rUreB, rHpaA, rVacA, rCagA1, rNapA, rFlaA, rFlaB, respectively; Lanes 1b-7b: blank controls.

Figure 4 PCR products of 7 genes of H pylori. M: Marker (BioColor); Lanes 1-7: ureB, hpaA, vacA, CagA, napA, flaA and flab genes of H pylori NCTC 11637, respectively.