Proliferation of L02 human hepatocytes in tolerized genetically immunocompetent rats

Figure 1 Fluorescence image of DiI-stained L02 hepatocytes (× 200). A-D: The Rts group at wk 1, 4, 8 and mo 6 after transplantation; E-H: The 2AAF group at wk 1, 4, 8 and mo 6 after transplantation.

Figure 2 L02 hepatocytes giving off green fluorescence in the rat liver tissue with chimeric human hepatocytes at wk 4 after transplantation (× 400). A: Normal rat liver tissue; B: The Rts group; C: The 2AAF group; D: The control.

Figure 3 L02 hepatocytes with brown nuclei in normal rat liver tissue and rat liver tissue with chimeric human hepatocytes at wk 4 after transplantation (× 200). A: Normal rat liver tissue; B: The Rts group; C: The 2AAF group; D: The control; E: Primary antibody not specifically against human PCNA.

Figure 4 Curve chart of number density of PCNA-positive cells number density of PCNA-positive cells. Using the primary antibody specifically against human PCNA, there were significant differences in the number density between the Rts and the control group, there were no significant differences between the 2AAF and the control group, while using the primary antibody not specifically against human PCNA, there were no significant differences between either groups.

Figure 5 RT-PCR detection of human and rat albumin mRNA in liver tissue. 1: Standard molecular weight DNA marker; 2, 3: Normal rat liver tissue; 4, 5: Human liver tissue; 6, 7: Rats liver tissue with chimeric human hepatocytes at wk 2, 4, 6, 8, and mo 4, 6 in the Rts group and at wk 2, 4, 6, 8 in the 2AAF and the control group; 2, 4, 6: Rat albumin mRNA primers used in the RT-PCR reaction system, amplified 388 bp fragment of rat albumin mRNA presented in the rat liver tissue of 2 (normal rat liver tissue) and 6 (rats liver tissue with chimeric human hepatocytes), but not in 4 (human liver tissue); 3, 5, 7: Human albumin mRNA primers used in the RT-PCR reaction system, the amplified products did not present in 3 (normal rat liver tissue), but in 5 (human liver tissue) and 7 (rats liver tissue with chimeric human hepatocytes), amplified 241 bp human albumin mRNA presented in the rat liver tissue.