Screening and cloning for proteins transactivated by the PS1TP5 protein of hepatitis B virus: A suppression subtractive hybridization study

Figure 1 PS1TP5 RT-PCR product electrophoresis on 10 g/L agarose gel. Lane 1: PS1TP5 PCR products; lane 2: DNA marker 2000.

Figure 2 Digestion and identification of the recombinant plasmid pGEM-T-PS1TP5 by EcoRI/BglII. Lane 1: Restriction analysis of pGEM-T-PS1TP5; lane 2: DNA marker 2000.

Figure 3 Restriction analysis of the recombinant plasmid pcDNA-3. 1(-)-myc-his(A)-PS1TP5 by EcoRI/BglII. Lane 1: Restriction analysis of pcDNA-3.1(-)-myc-his(A)-PS1TP5; lane 2: DNA marker 15 000.

Figure 4 PCR analysis of substraction efficiency. M: DNA marker 2000; lanes 1-4: PCR of 18, 23, 28, 33 cycles in unsubstracted group, respectively; lanes 5-8: PCR of 18, 23, 28, 33 cycles in substracted group, respectively.

Figure 5 PCR amplification of part of clones (56-63) in substracted cDNA library. M: DNA marker 2000.

Figure 6 The nucleotide sequence of PS1TP5TP1 gene and relevant amino acid sequences (GenBank accession number: DQ487761).