Selective inhibition of cell growth by activin in SNU-16 cells

Figure 1 Effects of activin A on cell viability in SNU-5 and SNU-16 cells. Cells (5 × 10⁴ cells/mL) were cultured with activin A (100 ng/mL) in a time-dependent manner and viable cells were counted with a hemocytometer. Values are the mean ± SD of three individual experiments. ^bP < 0.01 vs the control values.

Figure 2 mRNA expressions of inhibin/activin subunits, activin receptors, Smads, and p21^CIP1/WAF1 in human gastric cancer cell lines. The mRNA levels were analyzed by RT-PCR. M: DNA size marker; P: positive control; lane 1: AGS; lane 2: KATO III; lane 3: SNU-1; lane 4: SNU-5; lane 5: SNU-16; lane 6: SNU-484; lane 7: SNU-601; lane 8: SNU-638; lane 9: SNU-668; lane 10: SNU-719. Positive control was used for inhibin/activin subunits (mouse ovary), activin receptor (K562), Smad and p21^CIP1/WAF1 (human keratinocytes) respectively.

Figure 3 Effects of activin A on activin receptor mRNA expression (A), smad mRNA expression (B), and p21^CIP1/WAF1 mRNA expression (C) in SNU-16 cells. Cells (5 × 10⁴ cells/mL) were cultured with activin A (100 ng/mL) in a time-dependent manner and mRNA levels were measured by RT-PCR. Values are mean ± SD of three individual experiments and reported as the ratio of activin receptors to β-actin signals, the ratio of Smad to β-actin signals, and the ratio of p21^CIP1/WAF1 to β-actin signals, respectively. ^aP < 0.05 and ^bP < 0.01 vs the control values.