VP22 fusion protein-based dominant negative mutant can inhibit hepatitis B virus replication

Figure 1 Plasmid constructs used in the experiment. (Dotted lines represent the deleted sequences. The nomenclature of plasmids is listed in the right. The primers, US, UR, DS, and DR, are marked with arrows. Restriction enzymes are expressed in single letters. K1, KpnI; B1, BamHI; B2, BglII; X1, XhoI. Downstream sequence of 102 bp is the PTD of VP22).

Figure 2 Confirmation of transgene expression in HepG2. 2.15 cells.

Figure 3 Comparison of HBsAg (A) and HBV-DNA (B) concentrations between transfection groups.