Effect of deleted pancreatic cancer locus 4 gene transfection on biological behaviors of human colorectal carcinoma cells

Figure 1 Strongest expression of Smad4 protein in clone2 detected by Western blot analysis.

Figure 2 Expression of smad4 protein. A: Weak intracellular expression of Smad4 protein in SW620 cells. The positive staining for Smad4 was localized in cytoplasm; B: Weak intracellular expression of Smad4 protein in PcDNA3.1-SW620 cells. The positive staining for Smad4 was localized in cytoplasm; C: Strong intracellular expression of Smad4 protein in DPC4⁺-SW620 cells (clone2). The positive staining for Smad4 was localized in cytoplasm and nucleus, mainly in cytoplasm.

Figure 3 Growth property of different groups. A: Growth curve of cells of different groups; B: Doubling time of cells of different groups; C: Cloning efficiencies in different groups. ^bP<0.01 vs DPC4⁺-SW620.

Figure 4 Alterations of S% (A) and apoptosis rate (B) detected by flow- cytometry. ^aP<0.05 vs DPC4⁺-SW620.