Copyright
©The Author(s) 2005.
World J Gastroenterol. Jul 14, 2005; 11(26): 4094-4097
Published online Jul 14, 2005. doi: 10.3748/wjg.v11.i26.4094
Published online Jul 14, 2005. doi: 10.3748/wjg.v11.i26.4094
Figure 1 Agarose gel electrophoresis of caspase-12 RT-PCR product.
Lane 1: DL 2000 DNA Marker; lane 2: The 866 bp caspase-12 gene segment produced by RT-PCR.
Figure 2 In vitro transcription product of caspase-12 segment, separated by 6% PAGE, the transcript was 910 nt.
Figure 3 Agarose gel electrophoresis of PCR amplified ribozyme templates.
Lane 1: DL 2000 DNA Marker; lane 2: Rz138 template produced by PCR; lane 3: Rz218 template produced by PCR.
Figure 4 In vitro Cleavage of mouse caspase-12 mRNA by ribozymes.
A: The length of caspase-12 segment transcribed from BamHI linearized pCaspase-12 which contains the mouse caspase-12 gene segment, and it’s anticipated cleavage products. B: The results of cleavage reaction by PAGE electrophoresis. Lane 1: Rz138 cleavage result; lane 2: Rz218 cleavage result; lane 3: control (control contains all other components except ribozyme.).
- Citation: Jiang S, Xie Q, Zhang W, Zhou XQ, Jin YX. Preparation of anti-mouse caspase-12 mRNA hammerhead ribozyme and identification of its activity in vitro. World J Gastroenterol 2005; 11(26): 4094-4097
- URL: https://www.wjgnet.com/1007-9327/full/v11/i26/4094.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i26.4094