Effects of extracellular iron concentration on calcium absorption and relationship between Ca2+ and cell apoptosis in Caco-2 cells

Figure 1 Epithelial cells in primary culture. A: Ingredients in the sediment after centrifugation (×200); B: colonies formed in cells cultured for 6 d (×100); C: pavement-like cells formed in cells cultured for 9 d (×200); D: immune positive cells (↑) (×400); E: no staining in negative group (×100); F: cell movement, adherent cells (▲) and just adherent cells (↓) (×100).

Figure 2 Effect of different concentrations of DFO (A), FAC (B), and A₂₃₁₈₇ (C) on Ca²⁺ concentration in Caco-2 cells. A: 1a: Control, ×200; 1b: the final consistence of DFO was 100 μmol/L, ×200; 1c: the final consistence of DFO was 200 μmol/L, ×200; 1d: the final consistence of DFO was 300 μmol/L, ×200. B: 2a: control, ×200; 2b: the final consistence of FAC was 10 μmol/L, ×200; 2c: the final consistence of FAC was 50 μmol/L, ×200; 2d: the final consistence of FAC was 100 μmol/L, ×200. C: 3a (1) the photograph of transmission light, showing the place of cells, ×200; (2) the photograph of fluorescence, showing the active cells, ×200; 3b: blank control, ×200; 3c: negative control, ×200; 3d: the final consistence of A₂₃₁₈₇ was 0.1 μmol/L, ×200; 3e: the final consistence of A₂₃₁₈₇ was 1.0 μmol/L, ×200; 3f: the final consistence of A₂₃₁₈₇ was 10 μmol/L, ×200.