Viral Hepatitis
Copyright ©The Author(s) 2004.
World J Gastroenterol. Mar 15, 2004; 10(6): 841-846
Published online Mar 15, 2004. doi: 10.3748/wjg.v10.i6.841
Figure 1
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Figure 1 Enzymatic digestion of pBR322-2HBV by EcoRI.
Figure 2
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Figure 2 Enzymatic digestion of pcDNA3 by EcoRI. .
Figure 3
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Figure 3 Analysis of ligation of HBV and pcDNA3. 1, 3: pcDNA3 vector, 2: pcDNA3-HBV (5.4 kb + 3.2 kb), 4: DNA marker (λDNA/HindIII).
Figure 4
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Figure 4 Digestive identification of recombinant plasmid pcDNA3-HBV. 1: DNA marker (λDNA/HindIII), 2: XhoI di-gestion(3.1 kb + 5.5 kb), 3: Hind III digestion (8.6 kb), 4: EcoRI digestion (5.4 kb + 3.2 kb).
Figure 5
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Figure 5 Expression of preS1 mRNA by RT-PCR. 1: HepG2.2.15 (positive control); 2: β-actin; 3: HepG2.02G; 4: DNA marker (DL2 000); 5: HepG2 (negative control).
Figure 6
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Figure 6 Liver structure of mice by HE-staining (× 200). A: Liver structure of mice in blank control group, B: Liver structure of mice in negative control group.
Figure 7
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Figure 7 Pathological changes of mouse liver with acute hepatitis in model group(HE, ×200). A: Infiltration of inflammationary cells in the portal area, B: Local necrosis as arrow indicated, C: Obvious infiltration of inflammatory cells and eosinophilic changes, D: Cytoplasmic loosening of liver cells.