Primary hepatocyte culture in collagen gel mixture and collagen sandwich

Figure 1 Morphology of rat hepatocytes in collagen gel mix-ture culture at 24 h (phase-contrast microscope ×200).

Figure 2 Morphology of rat hepatocytes in collagen gel mix-ture culture at 72 h (phase-contrast microscope ×200).

Figure 3 Morphology of piglet hepatocytes cultured on a single layer of collagen gel at 4 h (phase-contrast microscope ×200).

Figure 4 Morphology of piglet hepatocytes cultured in sand-wich configurations on d 5 (phase-contrast microscope ×200).

Figure 5 BUN level in supernatant of medium.

Figure 6 Albumin secretion in cultured piglet hepatocytes by SDS-PAGE analysis. M: Low-range protein molecular weight markers; lanes A, B, C, D are culture media on days 1, 3, 5, 7.