Cloning and sequencing of cagA gene fragment of Helicobacter pylori with coccoid form

Figure 1 10 g/L agarose gel electrophoresis of cagA DNA fragment amplified by PCR from coccoidal H pylori. Lane 1. PCR products, Lane M: λ -Hind III DNA marker.

Figure 2 Identification of recombinant vector by PCR. Lane m: λ -Hind III DNA marker; Lane 1: amplification cagA gene from recombinant pMD-18T-cagA plasmid by PCR; Lane 2: Amplifi-cation cagA gene from coccoid H pylori genome DNA by PCR.

Figure 3 The identification of the pMD-18T-cagA by digestion with restriction endonucleases Lane1: Recombinant pMD-18T-cagA digested by BamH I puls Sac I; Lane 2: Amplification cagA gene from coccoid H pylori genome DNA by PCR; m: λ -Hind III DNA marker.

Figure 4 sequencing result of partial cagA gene.