Colorectal Cancer
Copyright ©The Author(s) 2004.
World J Gastroenterol. Dec 1, 2004; 10(23): 3459-3463
Published online Dec 1, 2004. doi: 10.3748/wjg.v10.i23.3459
Figure 1
Figure 1 Chemical structure of honokiol (C18H18O2, MW = 266. 33).
Figure 2
Figure 2 Concentration- and time-dependent inhibition of RKO cells exposed to honokiol shown by MTT assay. (A) RKO cells were plated in quadruplicate in 96-well plates and treated with increasing concentrations of honokiol for 68 h. (B) RKO cells treated with 5, 10 and 15 μg/mL of honokiol were tested at different time points.
Figure 3
Figure 3 Differences in vehicle or honokiol induced apoptotic DNA laddering of RKO cells. lane 1: control; lane 2: 5 μg/mL; lane 3: 10 μg/mL; lane 4: 15 μg/mL.
Figure 4
Figure 4 Apoptosis of RKO cells detected by FCM. (A) control; (B) 5 μg/mL; (C) 10 μg/mL; (D) 15 μg/mL.
Figure 5
Figure 5 Western blot analysis for the expression of Caspase-3 and -9 in human colorectal carcinoma cell line RKO cells. Lane 1: vehicle; lane 2: 5 μg/mL; lane 3: 10 μg/mL.
Figure 6
Figure 6 Cytocidal effect of honokiol on the growth of primary cultured human umbilical vein endothelial cells, primary hu-man fibroblast cells and human lymphocytes.
Figure 7
Figure 7 Honokiol concentration in plasma of BALB/c mice.
Figure 8
Figure 8 Effect of honokiol on the growth of xenografted RKO in Balb/c nude mice. The Y-axis represents tumor volume, X-axis represents time (day) after RKO cell inoculation.