Construction of a targeting adenoviral vector carrying AFP promoter for expressing EGFP gene in AFP producing hepatocarcinoma cell

Math 1 Math(A1).

Figure 1 Human 0. 3 kb AFP promoter.

Figure 2 Plasmid pshuttle and PCR product doubly-digested with MluI/NheI. M: marker, 1: the PCR product of 99-744 re-gion of pshuttle, 2: the product of PCR doubly digested with MluI/NheI, 3: pshuttle, 4: doubly-digested pshuttle with MluI/ NheI, a 670 bp fragment was released from pshuttle.

Figure 3 A: Transcription levels of EGFP shown by Northern blot in three different cell lines, B: Transcription levels of EGFP gene detected by semi-quantity analysis in three cell lines. Lane 1, HeLa cell; lane 2, HepG2 cell; lane 3, LO2 cell.

Figure 4 Expression of EGFP mRNA in HepG2 cells (A) and LO2 cells (B) ×200.