Brief Reports
Copyright ©The Author(s) 2004.
World J Gastroenterol. Sep 1, 2004; 10(17): 2560-2562
Published online Sep 1, 2004. doi: 10.3748/wjg.v10.i17.2560
Figure 1
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Figure 1 BabA2 DNA fragment amplified by PCR from Helicobacter pylori electrophoresed on 8 g/L agarose gel. Lane 1: Nucleotide marker; Lane 2: PCR products.
Figure 2
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Figure 2 Identification of recombinant plasmid by restriction enzyme digestion. Lane 1: Nucleotide marker; Lane 2: PCR products; Lane 3: Recombinant plasmid/Not I; Lane 4: Recombinant plasmid/Not I and Nco I; Lane5: pET22b (+)/Not I.
Figure 3
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Figure 3 The 100 g/L SDS-PAGE analysis of fusion protein expressed in BL21 (DE3). Lane 1: Molecular mass marker (67, 94) × 103; Lane 2: control strain BL21 (pET) before induction; Lane 3: control strain BL21 (pET) after 5-h induction with IPTG; Lane 4: BL21 (pET-BabA) cells before induction; Lane 5: BL21 (pET- BabA) cells after 3-h induction with IPTG; Lane 6: BL21 (pET- BabA) cells after 5-h induction with IPTG; Lane 7: BL21 (pET- BabA) cells periplasm protein after 3-h induction with IPTG; Lane 8: sonicated supernatant of BL21 (pET-BabA) cells after 3-h induction with IPTG; Lane 9: recombinant protein BabA purified.