Bai Y, Zhang YL, Chen Y, Jin JF, Zhang ZS, Zhou DY. Cloning and expression and immunogenicity of Helicobacter pylori BabA2 gene. World J Gastroenterol 2004; 10(17): 2560-2562 [PMID: 15300906 DOI: 10.3748/wjg.v10.i17.2560]
Corresponding Author of This Article
Dr. Yang Bai, PLA Institute for Digestive Medicine, Nanfang Hospital, the First Military Medical University, Guangzhou 510515, Guangdong Province, China. Baiyang1030@hotmail.com
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Brief Reports
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World J Gastroenterol. Sep 1, 2004; 10(17): 2560-2562 Published online Sep 1, 2004. doi: 10.3748/wjg.v10.i17.2560
Cloning and expression and immunogenicity of Helicobacter pylori BabA2 gene
Yang Bai, Ya-Li Zhang, Ye Chen, Jian-Feng Jin, Zhao-Shan Zhang, Dian-Yuan Zhou
Yang Bai, Ya-Li Zhang, Ye Chen, Dian-Yuan Zhou, PLA Institute for Digestive Medicine, Nanfang Hospital, the First Military Medical University, Guangzhou 510515, Guangdong Province, China
Jian-Feng Jin, Chemistry university of Beijing, Beijing 100071, China
Zhao-Shan Zhang, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No.30270078
Correspondence to: Dr. Yang Bai, PLA Institute for Digestive Medicine, Nanfang Hospital, the First Military Medical University, Guangzhou 510515, Guangdong Province, China. Baiyang1030@hotmail.com
Telephone: +86-20-61641532
Received: August 6, 2003 Revised: October 4, 2003 Accepted: October 7, 2003 Published online: September 1, 2004
Abstract
AIM: To construct a recombinant strain which expresses BabA of Helicobacter pylori (H pylori) and to study the immunogenicity of BabA.
METHODS: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E.coli strain. Furthermore, BabA immunogenicity was studied by animal test.
RESULTS: DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by GenBank. The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize rBabA.
CONCLUSION: BabA recombinant protein may be an potential vaccine for control and treatment of H pylori infection.
