Honokiol induces apoptosis through p53-independent pathway in human colorectal cell line RKO

Figure 1 Chemical structure of honokiol (C₁₈H₁₈O₂, M_r 266. 33).

Figure 2 Differences in vehicle and honokiol induced apoptotic DNA laddering of RKO cells. Lane 1: Vehicle; Lane 2: 5 μg/mL; Lane 3: 10 μg/mL.

Figure 3 Apoptosis of RKO cells detected by FCM. A: Vehicle; B: 5 μg/mL honokiol; C: 10 μg/mL honokiol; D: 15 μg/mL honokiol.

Figure 4 Analysis of Bcl- 2, Bax, Bid and Bcl- xl mRNA by RT-PCR. M: DNA mark; Lane 1: Vehicle; Lane 2: 5 μg/mL honokiol; Lane 3: 10 μg/mL honokiol.

Figure 5 Western blotting of honokiol- and vehicle-induced Bax, Bcl-2, Bcl-xl, Bid and p53 protein levels in RKO cell line. Lane 1: Vehicle; Lane 2: 5 μg/mL; Lane 3: 10 μg/mL.

Figure 6 Concentration-dependent inhibition of RKO, SW480 and LS180 cells exposed to the various concentrations of honokiol shown by MTT assay.