Effects of fucosylated milk of goat and mouse on Helicobacter pylori binding to Lewis b antigen

Figure 1 Formation of fucosylated blood group antigens.

Figure 2 Map of expression vector: pBC1-fut.

Figure 3 Blocking effect on H pylori binding to Lewis b antigen by goat milk. Abscissa: Goat milk collected at different time points and control milk. Ordinate: Rate of H pylori binding to Lewis b antigen.

Figure 4 PCR results of transgenic mice. M: DNA ladder; P: pBC1-fut plasmid; CK: Non-transgenic mouse. 15, 42, 47, 62 and 71: Serial numbers of gene positive mice.

Figure 5 Southern blotting of transgenic mice. M: DNA ladder; CK: Non-transgenic mouse; P1, P2, P5 and P10: pBC1-fut plas-mid equivalent to 1, 2, 5 and 10 gene copies, respectively. 15, 42, 47, 62 and 71: Serial numbers of gene positive mice.

Figure 6 Western blotting of the transient expression milk of goat. Lanes 1-8: Milk collected at different time points of 6, 18, 30, 42, 60, 78, 90, 100 h postinjection, respectively.