Growth inhibition and apoptosis induction of tanshinone II-A on human hepatocellular carcinoma cells

Figure 1 Growth-inhibitory effect of Tan II-A on SMMC-7721 cells detected by MTT. A: In a dose-dependent manner detected by MTT; B: In a time-dependent manner detected by MTT.

Figure 2 Morphological characteristics of SMMC-7721 cells before and after Tan II-A treatment. A: Roundish, large and serried SMMC-7721cells (in control group); B: Polygonal, small, detached or sparse, membranous frothed and wizened SMMC-7721cells (II A).

Figure 3 Ultrastructural changes of SMMC-7721 cells with 1. 0 mg/L Tan II-A treatment for 96 h under TEM (× 10000). The chromatin condensation, original margination were observed.

Figure 4 DNA agarose gel electrophoresis of SMMC-7721 cells with Tan II-A treatment for 96 h. Lane 1: Marker, Lane 2: Control, Lane 3: 2.0 mg.L^-1, Lane 4: 1.0 mg.L^-1, Lane 5: 0.5 mg.L^-1.

Figure 5 Flow cytometry analysis of apoptosis in SMMC-7721 cells treated with Tan II-A (A) and DMSO (B).