Cloning and identification of NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A

Figure 1 Western blotting of NS5A protein expression in HepG2 cells. Lane 1: Expression plasmid pcDNA3.1 (-)-NS5A; Lane 2: Plasmid pcDNA3.1 (-)-vector.

Figure 2 Transactivation on SV40 promoter by NS5A. 1: Plasmid pcDNA3.1 (-)-NS5A was cotransfected with pCAT3-promoter in HepG2 cells. 2: Plasmid pcDNA3.1 (-) was cotransfected with pCAT3-promoter in HepG2 cells.

Figure 3 Reduction of G3PDH amount by PCR-selective subtraction. PCR was performed on unsubtracted (Lanes 1-4) and subtracted (Lanes 5-8) secondary PCR products with the G3PDH 5’ and 3’ primers. Lanes 1, 5: 18 cycles; Lanes 2,6: 23 cycles; Lanes 3,7: 28 cycles.

Figure 4 Map of colony PCR on 9 g/L agarose/EtBr gel.

Figure 5 NS5A-TP2 fragment amplified by RT-PCR. M: Marker.

Figure 6 pEGM-T-NS5A-TP2 cut by EcoR I/Kpn I. M: Marker; Lane 1: A 512-bp fragment; Lane 2: A 615-bp fragment.

Figure 7 Comparison of exons and introns of NS5A-TP2 (615) and (512) gene.

Figure 8 ORF comparison of NS5A-TP2 (615) and (512).