Yang Q, Cheng J, Liu Y, Hong Y, Wang JJ, Zhang SL. Cloning and identification of NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A. World J Gastroenterol 2004; 10(12): 1735-1739 [PMID: 15188496 DOI: 10.3748/wjg.v10.i12.1735]
Corresponding Author of This Article
Dr. Jun Cheng, Gene Therapy Research Center, Institute of Infectious Diseases, 302 Hospital of PLA, 100 Xisihuanzhong Road, Beijing 100039, China. cj@genetherapy.com.cn
Article-Type of This Article
Viral Hepatitis
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This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Jun 15, 2004; 10(12): 1735-1739 Published online Jun 15, 2004. doi: 10.3748/wjg.v10.i12.1735
Cloning and identification of NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A
Qian Yang, Jun Cheng, Yan Liu, Yuan Hong, Jian-Jun Wang, Shu-Lin Zhang
Qian Yang, Shu-Lin Zhang, Department of Infectious Disease, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Jun Cheng, Yan Liu, Yuan Hong, Jian-Jun Wang, Gene Therapy Research Center, Institute of Infectious Diseases, 302 Hospital of PLA, 100 Xisihuanzhong Road, Beijing 100039, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. C03011402, No. C30070690 and the 9th Five-year Plan Period Research and Development Foundation of PLA, No. 98D063 and the Start-up for Students Studying Overseas of PLA, No. 98H038 and the 10th Five-year period Youth Research and Technology Foundation of PLA, No. 01Q138 and the 10th Five-year period Research and Technology Foundation of PLA, No. 01MB135
Correspondence to: Dr. Jun Cheng, Gene Therapy Research Center, Institute of Infectious Diseases, 302 Hospital of PLA, 100 Xisihuanzhong Road, Beijing 100039, China. cj@genetherapy.com.cn
Telephone: +86-10-66933391 Fax: +86-10-63801283
Received: November 21, 2003 Revised: December 22, 2003 Accepted: December 29, 2003 Published online: June 15, 2004
Abstract
AIM: To clone, identify and study new NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A.
METHODS: On the basis of subtractive cDNA library of genes transactivated by NS5A protein of hepatitis C virus, the coding sequence of new gene and its spliced variant were obtained by bioinformatics method. Polymerase chain reaction (PCR) was conducted to amplify NS5ATP2 gene.
RESULTS: The coding sequence of a new gene and its spliced variant were cloned and identified successfully.
CONCLUSION: A new gene has been recognized as the new target transactivated by HCV NS5A protein. These results brought some new clues for studying the biological functions of new genes and pathogenesis of the viral proteins.
