Randomized Controlled Trial
Copyright ©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 28, 2025; 31(12): 104081
Published online Mar 28, 2025. doi: 10.3748/wjg.v31.i12.104081
Randomized study of Lacticaseibacillus fermented milk in Indonesian elderly houses: Impact on gut microbiota and gut environment
I Nengah Sujaya, Mariyatun Mariyatun, Pratama Nur Hasan, Nancy Eka Putri Manurung, Putrika Citta Pramesi, Mohammad Juffrie, Tyas Utami, Muhammad Nur Cahyanto, Shuta Yamamoto, Takuya Takahashi, Takashi Asahara, Takuya Akiyama, Endang Sutriswati Rahayu
I Nengah Sujaya, School of Public Health, Faculty of Medicine, Udayana University, Denpasar 80230, Bali, Indonesia
Mariyatun Mariyatun, Pratama Nur Hasan, Nancy Eka Putri Manurung, Putrika Citta Pramesi, Endang Sutriswati Rahayu, Center for Food and Nutrition Studies, Universitas Gadjah Mada, Sleman 55281, Daerah Istimewa Yogyakarta, Indonesia
Mariyatun Mariyatun, Pratama Nur Hasan, Nancy Eka Putri Manurung, Putrika Citta Pramesi, Tyas Utami, Endang Sutriswati Rahayu, Center of Excellence for Research and Application on Integrated Probiotics Industry, Universitas Gadjah Mada, Sleman 55281, Daerah Istimewa Yogyakarta, Indonesia
Putrika Citta Pramesi, Tyas Utami, Muhammad Nur Cahyanto, Endang Sutriswati Rahayu, Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Sleman 55281, Daerah Istimewa Yogyakarta, Indonesia
Mohammad Juffrie, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Sleman 55281, Daerah Istimewa Yogyakarta, Indonesia
Shuta Yamamoto, Takashi Asahara, Takuya Akiyama, Yakult Central Institute, Yakult Honsha Co., Ltd., Kunitachi 186-8650, Tōkyō, Japan
Takuya Takahashi, Yakult Honsha European Research Center for Microbiology VOF, Ghent 9052, East Flanders, Belgium
Author contributions: Sujaya IN, Mariyatun M, Hasan PN, Manurung NEP, Pramesi PC, Yamamoto S, Takahashi T, and Asahara T contributed to the investigation of this manuscript; Sujaya IN, Cahyanto MN, and Rahayu ES were involved in the supervision of this manuscript; Sujaya IN and Rahayu ES contributed to the validation of this manuscript; Sujaya IN wrote the original draft; Mariyatun M, Hasan PN, and Akiyama T performed formal analysis; Mariyatun M and Akiyama T contributed to the data curation; Mariyatun M and Hasan PN participated in the resources; Mariyatun M, Juffrie M, Utami T, Yamamoto S, Takahashi T, Asahara T, Akiyama T, and Rahayu ES contributed to the methodology of this manuscript; Mariyatun M performed project administration and funding acquisition; Mariyatun M, Pramesi PC, and Akiyama T were involved in the writing - review and editing of this manuscript; Manurung NEP contributed to the data curated; Juffrie M, Utami T, Cahyanto MN, and Rahayu ES responsible in the conceptualization of this manuscript; Akiyama T contributed to the software.
Supported by the Yakult Honsha Co., Ltd., No. 1226/FTP-UGM/HK/2018.
Institutional review board statement: This study was approved by the Medical and Health Research Ethics Committee, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, with reference number KE/FK/0202/EC/2018 (approval date: March 16, 2018) and an amendment with reference number KE/FK/0202/EC/2018 (approval date: September 13, 2018).
Clinical trial registration statement: This study was registered at ClinicalTrials.gov. The registration number is NCT05308745.
Informed consent statement: All study participants or their legal guardians provided written informed consent before study enrollment.
Conflict-of-interest statement: Yamamoto S, Takahashi T, Asahara T, and Akiyama T are affiliated with Yakult Honsha Co., Ltd. The authors declare that the research was conducted in the absence of commercial or financial relationships that could be construed as potential conflicts of interest.
CONSORT 2010 statement: The authors have read the CONSORT 2010 Statement, and the manuscript was prepared and revised according to the CONSORT 2010 Statement.
Data sharing statement: The next-generation sequencing data from this study are publicly available through the DDBJ Sequence Read Archive under accession number DRA014200.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Endang Sutriswati Rahayu, PhD, Professor, Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1 Bulaksumur, Sleman 55281, Daerah Istimewa Yogyakarta, Indonesia. endangsrahayu@ugm.ac.id
Received: December 10, 2024
Revised: January 21, 2025
Accepted: February 24, 2025
Published online: March 28, 2025
Processing time: 107 Days and 4.2 Hours
Core Tip

Core Tip: The daily consumption of fermented milk containing Lacticaseibacillus paracasei Shirota significantly altered the gut microbiota among individuals in Indonesian elderly houses. Notably, these changes included increases in Bifidobacterium and Succinivibrio and a decrease in the Rikenellaceae RC9 gut group. These modifications are associated with an improvement in the gut environment, as indicated by elevated butyrate levels.