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©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 14, 2024; 30(42): 4532-4543
Published online Nov 14, 2024. doi: 10.3748/wjg.v30.i42.4532
Published online Nov 14, 2024. doi: 10.3748/wjg.v30.i42.4532
Strategic insights into the cultivation of pancreatic cancer organoids from endoscopic ultrasonography-guided biopsy tissue
Jia-Li Yang, Jun-Feng Zhang, Jian-You Gu, Mei Gao, Ming-You Zheng, Shi-Xiang Guo, Tao Zhang, Institute of Hepatopancreatobiliary Surgery, Chongqing General Hospital, Chongqing Uni versity, Chongqing 401147, China
Co-first authors: Jia-Li Yang and Jun-Feng Zhang.
Co-corresponding authors: Shi-Xiang Guo and Tao Zhang.
Author contributions: Yang JL and Zhang JF designed study, conducted experiment, collected the data and wrote article; Gu JY contributed to the analysis; Gao M guided the cultivation technique; Zheng MY provided clinical advice; Guo SX and Zhang T operated endoscopes, collected samples and revised article.
Supported by the Chongqing Talent Plan “Contract System” Project , No. cstc2022ycjh-bgzxm0137 ; and Natural Science Foundation of Chongqing , No. CSTB2024NSCQ-MSX0003 .
Institutional review board statement: This study was reviewed and approved by the Ethics Committee of Chongqing General Hospital. The ethics review number: No. KY S2022-045-01.
Clinical trial registration statement: The protocol registered at Chinese Clinical Trial Registry. The registration identification number: No. ChiCTR2200064388.
Informed consent statement: All participants or their legal guardian signed informed consent forms allowing their puncture samples and medical data to be used for scientific research.
Conflict-of-interest statement: The authors declare that there is no conflict of interest.
Data sharing statement: Datasets generated during the current study are available upon reasonable request.
CONSORT 2010 statement: The authors have read the CONSORT 2010 Statement, and the manuscript was prepared and revised according to the CONSORT 2010 Statement.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Tao Zhang, MM, Associate Professor, Institute of Hepatopancreato biliary Surgery, Chongqing General Hospital, Chongqing University, No. 118 Xingguang Avenue, Liangjiang New Area, Chongqing 401147, China. tzhang04@126.com
Received: May 6, 2024
Revised: September 10, 2024
Accepted: October 16, 2024
Published online: November 14, 2024
Processing time: 178 Days and 8.3 Hours
Revised: September 10, 2024
Accepted: October 16, 2024
Published online: November 14, 2024
Processing time: 178 Days and 8.3 Hours
Core Tip
Core Tip: We optimized the endoscopic ultrasound puncture process to obtain pancreatic cancer tissue and cultivate pan