Basic Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 7, 2024; 30(41): 4449-4460
Published online Nov 7, 2024. doi: 10.3748/wjg.v30.i41.4449
Amino acid deletions at positions 893 and 894 of cytotoxin-associated gene A protein affect Helicobacter pylori gastric epithelial cell interactions
Zhi-Jing Xue, Ya-Nan Gong, Li-Hua He, Lu Sun, Yuan-Hai You, Dong-Jie Fan, Mao-Jun Zhang, Xiao-Mei Yan, Jian-Zhong Zhang
Zhi-Jing Xue, Research Center of Translational Medicine, Central Hospital Affiliated to Shandong First Medical University, Jinan 250013, Shandong Province, China
Ya-Nan Gong, Li-Hua He, Lu Sun, Yuan-Hai You, Dong-Jie Fan, Mao-Jun Zhang, Xiao-Mei Yan, Jian-Zhong Zhang, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Author contributions: Xue ZJ and Zhang JZ designed the research study; Gong YN and He LH constructed the recombinant plasmids; Sun L, You YH, and Fan DJ performed the DNA extraction; Xue ZJ, Zhang MJ, and Yan XM analyzed the data and wrote the manuscript; Zhang JZ reviewed and revised the manuscript; and all authors have read and approved the final manuscript.
Supported by the Shandong Medical and Health Science and Technology Development Plan Project, No. 202202080452.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board of the National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention (approval No. ICDC-2013001).
Conflict-of-interest statement: The authors have no conflicts of interest and financial disclosures.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Jian-Zhong Zhang, MD, Professor, National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Changping District, Beijing 102206, China. zhangjianzhong@icdc.cn
Received: March 12, 2024
Revised: September 29, 2024
Accepted: October 12, 2024
Published online: November 7, 2024
Processing time: 225 Days and 4.1 Hours
Core Tip

Core Tip: The present study evaluated the impact of amino acid deletions at positions 893 and 894 on cytotoxin-associated gene A (CagA) protein function. Amino acid deletions at positions 893 and 894 enhance the ability of CagA to induce hummingbird cells, cytoskeletal rearrangement, and interleukin-8 secretion. This finding is crucial for elucidating the molecular mechanism of CagA protein pathogenicity and identifying biomarkers of highly pathogenic Helicobacter pylori.