Observational Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 14, 2023; 29(18): 2864-2874
Published online May 14, 2023. doi: 10.3748/wjg.v29.i18.2864
Endoscopic ultrasound-guided fine-needle aspiration pancreatic adenocarcinoma samples yield adequate DNA for next-generation sequencing: A cohort analysis
Stefania Bunduc, Bianca Varzaru, Razvan Andrei Iacob, Andrei Sorop, Ioana Manea, Andreea Spiridon, Raluca Chelaru, Adina Emilia Croitoru, Gabriel Becheanu, Mona Dumbrava, Simona Dima, Irinel Popescu, Cristian Gheorghe
Stefania Bunduc, Bianca Varzaru, Razvan Andrei Iacob, Gabriel Becheanu, Simona Dima, Cristian Gheorghe, Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest 020021, Romania
Stefania Bunduc, Razvan Andrei Iacob, Adina Emilia Croitoru, Gabriel Becheanu, Mona Dumbrava, Simona Dima, Irinel Popescu, Cristian Gheorghe, Digestive Diseases and Liver Transplantation Center, Fundeni Clinical Institute, Bucharest 022328, Romania
Stefania Bunduc, Bianca Varzaru, Razvan Andrei Iacob, Andrei Sorop, Ioana Manea, Andreea Spiridon, Raluca Chelaru, Adina Emilia Croitoru, Gabriel Becheanu, Mona Dumbrava, Simona Dima, Irinel Popescu, Cristian Gheorghe, Center of Excellence in Translational Medicine, Fundeni Clinical Institute, Bucharest 022328, Romania
Author contributions: Bunduc S contributed to conceptualization, methodology, investigation, formal analysis, original draft, and data visualization; Varzaru B contributed to conceptualization, investigation, data curation, methodology, review and editing; Iacob RA contributed to conceptualization, supervision, review and editing, methodology, funding acquisition, and resources; Sorop A contributed to methodology, investigation, data curation, review and editing; Manea I contributed to methodology, investigation, data curation, review and editing; Spiridon A contributed to investigation, data curation, review and editing; Chelaru R contributed to investigation, data curation, review and editing; Becheanu G contributed to conceptualization, methodology, investigation, review and editing; Dumbrava M contributed to conceptualization, methodology, investigation, review and editing; Croitoru A contributed to conceptualization, methodology, review and editing, Dima S contributed to conceptualization, supervision, funding acquisition, project administration, review and editing, resources; Popescu I contributed to conceptualization, supervision, funding acquisition, project administration, review and editing, Gheorghe C contributed to guarantor of the article, conceptualization, supervision, funding acquisition, project administration, review and editing; all authors certify that they have participated sufficiently in this work to take public responsibility for the content, including participation in the concept, design, analysis, writing, or revision of the manuscript. All authors have read the journal's authorship agreement, and reviewed and approved the final version of the manuscript.
Supported by The Executive Agency for Higher Education, Research, Development and Innovation Funding-research, No. PN-III-P1-1.2-PCCDI-2017-0797 (PANCNGS).
Institutional review board statement: This prospective observational study was approved by the Internal Review Board of Fundeni Clinical Institute, No. IRB:23878/14.06.2018.
Informed consent statement: Each participant gave informed consent for study enrolment.
Conflict-of-interest statement: All authors confirm they have no conflict of interest in relation to this manuscript.
Data sharing statement: The data underlying this article will be shared upon reasonable request to the corresponding author.
STROBE statement: The authors have read the STROBE Statement-checklist of items, and the manuscript was prepared and revised according to the STROBE Statement-checklist of items.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Cristian Gheorghe, FASGE, MD, PhD, Professor, Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, No. 258 Fundeni Road, Bucharest 020021, Romania. drcgheorghe@gmail.com
Received: December 31, 2022
Peer-review started: December 31, 2022
First decision: February 28, 2023
Revised: March 14, 2023
Accepted: April 18, 2023
Article in press: April 18, 2023
Published online: May 14, 2023
Core Tip

Core Tip: Genetic testing is increasingly undertaken in pancreatic adenocarcinoma (PDAC). The main diagnostic method is endoscopic ultrasound (EUS)-guided tissue acquisition and fine needle aspiration (FNA) is most frequently performed in clinical practice. However, current guidelines recommend using core specimens for genotyping aimed samples. In our cohort analysis, we show that EUS-FNA PDAC samples yield DNA of adequate amount and purity for subsequent next-generation sequencing (NGS). DNA amount was similar between 22G and 19G FNA needles. DNA purity parameters may vary indirectly with needle size. EUS FNA PDAC samples may be used for NGS.