Basic Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 7, 2022; 28(5): 532-546
Published online Feb 7, 2022. doi: 10.3748/wjg.v28.i5.532
Effect of Bacillus subtilis, Enterococcus faecium, and Enterococcus faecalis supernatants on serotonin transporter expression in cells and tissues
Yi-Ming Chen, Ying Li, Xin Wang, Ze-Lan Wang, Jun-Jie Hou, Shuai Su, Wei-Long Zhong, Xin Xu, Jie Zhang, Bang-Mao Wang, Yu-Ming Wang
Yi-Ming Chen, Ying Li, Xin Wang, Ze-Lan Wang, Jun-Jie Hou, Shuai Su, Wei-Long Zhong, Xin Xu, Jie Zhang, Bang-Mao Wang, Yu-Ming Wang, Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, Tianjin 300052, China
Author contributions: Wang YM, Wang BM and Zhang J conceived and designed the study; Chen YM and Wang ZL conducted the experiments; Li Y and Wang X rote the manuscript; Su S, Hou JJ, Zhong WL and Xu X analyzed data; Chen YM and Li Y contributed equally to this work; all authors read and approved the manuscript.
Supported by the National Natural Science Foundation of China, No. 81570489; and the Youth Project of National Natural Science Foundation of China, No. 81900487.
Institutional review board statement: This study was reviewed and approved by the Ethics Committee of Tianjin Medical University General Hospital (IRB2021-WZ-100).
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Institute of Radiation Medicine, Chinese Academy of Medical Sciences (Tianjin, China) (IACUC Protocol No. IRM-DWLL-2021142).
Conflict-of-interest statement: The authors have no conflicts of interest to declare.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Yu-Ming Wang, MD, Chief Doctor, Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin 300052, China. yumingwangbest@163.com
Received: July 21, 2021
Peer-review started: July 21, 2021
First decision: October 3, 2021
Revised: October 16, 2021
Accepted: January 6, 2022
Article in press: January 6, 2022
Published online: February 7, 2022
Processing time: 187 Days and 18.8 Hours
Core Tip

Core Tip: Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder that can cause chronic symptoms and changes in bowel habits. Serotonin and serotonin transporter (SERT) play significant roles in the development of IBS. Bacillus subtilis (B. subtilis), Enterococcus faecium (E. faecium), and Enterococcus faecalis (E. faecalis) are probiotics that are widely used in the clinical treatment of IBS. Here, we explored the effects of B. subtilis, E. faecium, and E. faecalis supernatants on the mRNA and protein expression of SERT in vitro and in vivo, highlighting the significance of the regulation of SERT expression in the treatment of IBS patients.