Basic Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 21, 2022; 28(43): 6109-6130
Published online Nov 21, 2022. doi: 10.3748/wjg.v28.i43.6109
Differential analysis of intestinal microbiota and metabolites in mice with dextran sulfate sodium-induced colitis
Jia-Li Wang, Xiao Han, Jun-Xiang Li, Rui Shi, Lei-Lei Liu, Kai Wang, Yu-Ting Liao, Hui Jiang, Yang Zhang, Jun-Cong Hu, Li-Ming Zhang, Lei Shi
Jia-Li Wang, Xiao Han, Hui Jiang, Yang Zhang, Jun-Cong Hu, Li-Ming Zhang, Graduate School, Beijing University of Chinese Medicine, Beijing 100029, China
Jia-Li Wang, Xiao Han, Jun-Xiang Li, Rui Shi, Hui Jiang, Yang Zhang, Jun-Cong Hu, Li-Ming Zhang, Lei Shi, Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
Lei-Lei Liu, College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China
Kai Wang, Department of Emergency, The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Yu-Ting Liao, Department of Geriatrics, Gulou Hospital of Traditional Chinese Medicine, Beijing 100009, China
Author contributions: Wang JL and Han X have contributed equally to this work, and they are co-first authors; Shi L and Li JX conceived and designed the study; Wang JL and Han X performed major experimental work; Wang JL and Shi R acquired and analyzed the results and edited the manuscript; Liu LL, Wang K, Liao YT, Jiang H, Zhang Y, Hu JC, and Zhang LM performed the experiments and statistical analyses; Shi L and Li JX revised the manuscript; All authors read and approved the final version of the manuscript.
Supported by the 13th Five-Year Plan for National Key R&D Program of China, No. 2018YFC1705405; Scientific Research Innovation Team Project of Beijing University of Chinese Medicine, No. 2019-JYB-TD004; New Faculty Startup Fund Program of BUCM, No. 2022-JYB-XJSJJ078; and National Natural Science Foundation of China, No. 82004113.
Institutional animal care and use committee statement: The animal study was reviewed and approved by the Animal Ethics Committee of Beijing University of Chinese Medicine. All animal experiments conformed to the internationally accepted principles for the care and use of laboratory animals (certificate No. SCXK-2019-0010, SPF Biotechnology Co., Ltd., Beijing, China; protocol No. BUCM-2020-01162, The Animal Ethics Committee of Beijing University of Chinese Medicine, Beijing, China).
Conflict-of-interest statement: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflict of interest.
Data sharing statement: All data are available upon reasonable request from LS, b01350@bucm.edu.cn.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Lei Shi, MD, PhD, Research Associate, Department of Gastroenterology, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6 Fangxingyuan Zone 1, Fangzhuang, Fengtai District, Beijing 100078, China. b01350@bucm.edu.cn
Received: July 12, 2022
Peer-review started: July 12, 2022
First decision: September 26, 2022
Revised: October 4, 2022
Accepted: November 2, 2022
Article in press: November 2, 2022
Published online: November 21, 2022
Processing time: 127 Days and 2.3 Hours
Core Tip

Core Tip: We observed that the differences in microbiota and their metabolites can cause different degrees of colitis. The differential metabolites of the microbiota in colitis are mainly enriched in pathways related to the key structural sequence synthesis of mucin, and the different levels of the metabolites lead to differential expression of mucin and mucus. Therefore, the differential metabolites of colitis could regulate the composition and function of mucus. In addition, the differential intestinal microbiota and their metabolites in mice with colitis were largely associated with amino acid and energy metabolism.