Basic Study
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 28, 2021; 27(32): 5404-5423
Published online Aug 28, 2021. doi: 10.3748/wjg.v27.i32.5404
Recombinant angiopoietin-like protein 4 attenuates intestinal barrier structure and function injury after ischemia/reperfusion
Zi-Yi Wang, Jian-Yu Lin, Yang-Rong Feng, De-Shun Liu, Xu-Zi Zhao, Tong Li, Si-Yuan Li, Jing-Chao Sun, Shu-Feng Li, Wen-Yan Jia, Hui-Rong Jing
Zi-Yi Wang, Emergent Intensive Care Unit, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China
Jian-Yu Lin, Department of Gastrointestinal Surgery, Weihai Central Hospital, Weihai 264200, Shandong Province, China
Yang-Rong Feng, Graduate College, Shandong First Medical University, Jinan 271000, Shandong Province, China
De-Shun Liu, Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027, Liaoning Province, China
Xu-Zi Zhao, Department of Pharmacology, Dalian Medical University, Dalian 116044, Liaoning Province, China
Tong Li, Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100000, China
Si-Yuan Li, Jing-Chao Sun, Shu-Feng Li, Hui-Rong Jing, Department of General Surgery, Qingdao Municipal Hospital, Qingdao University, Qingdao 266071, Shandong Province, China
Wen-Yan Jia, Physiological Examination Center, The Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong Province, China
Author contributions: Wang ZY, Jing HR and Lin JY designed the study; Jing HR and Wang ZY supervised the study; Liu DS, Zhao XZ and Sun JC collected and analyzed the data; Li SY and Feng YR performed the R Script; Lin JY and Li SF and Jia WY prepared the figures and drafted the manuscript; Jing HR and Li Tong revised the manuscript for important intellectual content; Jing HR and Jia WY contributed equally as co-corresponding authors; all authors read and approved the final manuscript.
Supported by the National Natural Science Foundation of China, No. 81600446; the Science and Technology of Traditional Chinese Medicine Foundation in Qingdao, No. 2021-zyyz03; and the Science and technology development of Medicine and health Foundation in Shandong Province, China, No. 202004010508.
Institutional animal care and use committee statement: The study was reviewed and approved by the Dalian Medical University Institutional Review Board (Approval No. L20190232).
Conflict-of-interest statement: The authors declare that they have no competing interests.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE Guidelines, and the manuscript was prepared and revised according to the ARRIVE Guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hui-Rong Jing, MD, PhD, Associate Professor, Surgeon, Department of General Surgery, Qingdao Municipal Hospital, Qingdao University, No. 5 Donghai Middle Road, Southern District, Qingdao 266071, Shandong Province, China. jhrdlmu_edu@126.com
Received: April 24, 2021
Peer-review started: April 24, 2021
First decision: June 3, 2021
Revised: June 17, 2021
Accepted: July 30, 2021
Article in press: July 30, 2021
Published online: August 28, 2021
Processing time: 122 Days and 19.2 Hours
Core Tip

Core Tip: Improving therapy on intestinal barrier dysfunction induced by ischemia/ reperfusion is still challenging. Most recently, studies indicated that patients who suffered from coronavirus disease 2019 are associated with intestinal hypoperfusion. We investigated the effect of recombinant human angiopoietin-like protein 4 on intestinal barrier structure and function deterioration using intestinal ischemia/ reperfusion models in rats as well as hypoxia/reoxygenation models in cells. Intestinal barrier indicators associated with the inflammatory and oxidative cascade, excessive activation of cellular autophagy and apoptosis were compared. Our results indicated that recombinant human angiopoietin-like protein 4 behaved as a promising therapeutic agent for intestinal ischemia/reperfusion-induced intestine injury.