Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 7, 2020; 26(9): 918-932
Published online Mar 7, 2020. doi: 10.3748/wjg.v26.i9.918
Kynurenine plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice
Chieko Tashita, Masato Hoshi, Akihiro Hirata, Kentaro Nakamoto, Tatsuya Ando, Takayuki Hattori, Yasuko Yamamoto, Hiroyuki Tezuka, Hiroyuki Tomita, Akira Hara, Kuniaki Saito
Chieko Tashita, Kentaro Nakamoto, Tatsuya Ando, Yasuko Yamamoto, Kuniaki Saito, Department of Disease Control and Prevention, Fujita Health University Graduate School of Health Sciences, Toyoake 470-1192, Japan
Masato Hoshi, Department of Biochemical and Analytical Science, Fujita Health University Graduate School of Health Sciences, Toyoake 470-1192, Japan
Akihiro Hirata, Division of Animal Experiment, Life Science Research Center, Gifu University, Gifu 501-1193, Japan
Tatsuya Ando, Hiroyuki Tezuka, Department of Cellular Function Analysis, Research Promotion and Support Headquarters, Fujita Health University Graduate School of Health Sciences, Toyoake 470-1192, Japan
Takayuki Hattori, Faculty of Medical Technology, Gifu University of Medical Science, Gifu 501-3892, Japan
Hiroyuki Tomita, Akira Hara, Department of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu 501-1193, Japan
Author contributions: Hoshi M, Hara A, and Saito K planned the studies; Tashita C, Hoshi M, Hirata A, Tezuka H, Hattori T, Yamamoto Y, and Tomita H performed the experiments; Tashita C and Hoshi M had responsibility for all data integrity and data analysis; Tashita C, Hoshi M, Hirata A, Tezuka H, Nakamoto K, Ando T, Hattori T, Yamamoto Y, Tomita H, Hara A, and Saito K discussed the results; Tashita C, Hoshi M, and Tezuka H wrote the manuscript; Hara A and Saito K conducted the research; Saito K had primary responsibility for the final content; all authors reviewed and approved the manuscript.
Supported by Grants-in-Aids for Young Scientists (B) from the Japan Society for the Promotion of Science, No. 17K15785; and Fujita Health University Grant (2018).
Institutional review board statement: This research was approved by the Ethics Committee of Fujita Health University.
Institutional animal care and use committee statement: This research was approved by Animal Experimental Ethical Inspection of Fujita Health University, NO. APU19040.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Masato Hoshi, PhD, Assistant Professor, Department of Biochemical and Analytical Science, Fujita Health University Graduate School of Health Sciences, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake 470-1192, Japan.
Received: November 5, 2019
Peer-review started: November 5, 2019
First decision: December 23, 2019
Revised: January 6, 2020
Accepted: February 21, 2020
Article in press: February 21, 2020
Published online: March 7, 2020
Core Tip

Core tip: The role of kynurenine 3-monooxygenase (KMO) in immune regulation was examined in KMO gene deficient mice suffering from 2,4,6-trinitrobenzene sulfate-induced colitis. We demonstrated that the expression of transforming growth factor-β and interleukin-10 in the colon of these mice was upregulated by KMO inhibition and kynurenine administration, resulting in increased incidence of regulatory T cells in the inflammatory site, where they suppress progression to colitis. Thus, administration of kynurenine plays a critical role in host protection during 2,4,6-trinitrobenzene sulfate-induced colitis.