Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 14, 2020; 26(46): 7299-7311
Published online Dec 14, 2020. doi: 10.3748/wjg.v26.i46.7299
Untargeted metabolomics characteristics of nonobese nonalcoholic fatty liver disease induced by high-temperature-processed feed in Sprague-Dawley rats
Li-Jun Xue, Ju-Qiang Han, Yuan-Chen Zhou, Hong-Ye Peng, Teng-Fei Yin, Kai-Min Li, Shu-Kun Yao
Li-Jun Xue, Shu-Kun Yao, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China
Ju-Qiang Han, Department of Hepatology, The Seventh Medical Center of PLA General Hospital, Beijing 100700, China
Yuan-Chen Zhou, Hong-Ye Peng, Teng-Fei Yin, Kai-Min Li, Shu-Kun Yao, Department of Gastroenterology, China-Japan Friendship Hospital, Beijing 100029, China
Author contributions: Xue LJ, Zhou YC, Peng HY and Yin TF performed the experiments; Xue LJ analyzed the data, prepared figures and contributed to the drafting of the manuscript; Yao SK and Han JQ supervised this work and edited and revised manuscript; Yao SK initiated the project, design the experiments and approved the final version of manuscript; Li KM provided technical assistance; All authors read and approved the final manuscript.
Supported by Science and Technology Project Task Book of Beijing, No. Z171100001717008.
Institutional animal care and use committee statement: The study was approved by the Animal Care Committees at China-Japan Friendship Hospital (Approval No: 190201).
Conflict-of-interest statement: All authors report no conflicts of interest.
Data sharing statement: Technical appendix, statistical code, and dataset available from the first author at
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Shu-Kun Yao, MD, PhD, Professor, Department of Gastroenterology, China-Japan Friendship Hospital, No. 2 Yinghua East Road, Chaoyang District, Beijing 100029, China.
Received: August 17, 2020
Peer-review started: August 17, 2020
First decision: October 18, 2020
Revised: October 27, 2020
Accepted: November 9, 2020
Article in press: November 9, 2020
Published online: December 14, 2020
Core Tip

Core Tip: For some patients with normal body mass index and normal serum indexes, liver damage may already exist. In our previous analysis of clinical data and a high-risk factor questionnaire for patients with nonalcoholic fatty liver disease (NAFLD), we found that the body mass index of some patients did not meet the diagnostic criteria for overweight or obesity. The consumption of high-temperature-processed foods such as fried food and hot pot is closely associated with the occurrence of NAFLD. Dietary intervention to reduce the consumption of such foods can alleviate NAFLD and improve prognosis. For patients with mild liver fat changes and early fibrosis, some clinical equipment and means of diagnosis are prone to misdiagnosis. Untargeted metabolomics can preliminarily explain the difference in liver pathology in the three groups of rats.