Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 14, 2020; 26(26): 3750-3766
Published online Jul 14, 2020. doi: 10.3748/wjg.v26.i26.3750
Adipose-derived mesenchymal stem cells alleviate TNBS-induced colitis in rats by influencing intestinal epithelial cell regeneration, Wnt signaling, and T cell immunity
Jian-Guo Gao, Mo-Sang Yu, Meng-Meng Zhang, Xue-Wei Gu, Yue Ren, Xin-Xin Zhou, Dong Chen, Tian-Lian Yan, You-Ming Li, Xi Jin
Jian-Guo Gao, Mo-Sang Yu, Meng-Meng Zhang, Xue-Wei Gu, Yue Ren, Xin-Xin Zhou, Tian-Lian Yan, You-Ming Li, Xi Jin, Department of Gastroenterology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China
Dong Chen, Department of Colorectal Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China
Author contributions: Gao JG and Yu MS contributed equally to this work, and they designed the study and drafted the manuscript; Zhang MM, Gu XW, Ren Y, and Zhou XX performed the study; Chen D conducted the statistical analysis; Zhang MM and Yan TL provided guidance during revision; Jin X and Li YM supervised the study and provided consultation during the entire study.
Supported by National Natural Science Foundation of China, No. 81770574, No. 81600414, and No. 81600447.
Institutional animal care and use committee statement: This study was carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The animal protocol was approved by the institutional review board of the First Affiliated Hospital of Zhejiang University and adhered to the standards articulated in Animal Research. The study was reviewed and approved by Animal Experimental Ethical Inspection of the First Affiliated Hospital, College of Medicine, Zhejiang University.
Conflict-of-interest statement: The authors have no conflicts of interest to disclose.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: Authors have read the ARRIVE guidelines and prepared and revised the manuscript according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Xi Jin, PhD, Associate Chief Physician, Department of Gastroenterology, The First Affiliated Hospital, School of Medicine, Zhejiang University, No. 79, Qingchun Road, Hangzhou 310003, Zhejiang Province, China. jxfl007@zju.edu.cn
Received: December 27, 2019
Peer-review started: December 27, 2019
First decision: February 14, 2020
Revised: May 14, 2020
Accepted: June 3, 2020
Article in press: June 3, 2020
Published online: July 14, 2020
Core Tip

Core tip: The prevalence and mortality of Crohn’s disease (CD) have been increasing globally, including in areas of Asia that previously had a low incidence. We aimed to investigate the effect and explore potential mechanisms of adipose-derived mesenchymal stem cells (ADSCs) in a 2,4,6-trinitrobenzene sulfonic acid-induced rat model of CD. Our study for the first time provided evidence that successfully isolated ADSCs show therapeutic effects in CD by regulating intestinal epithelial cell proliferation, the Wnt signaling pathway, and T cell immunity.