Copyright
©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 28, 2020; 26(20): 2570-2583
Published online May 28, 2020. doi: 10.3748/wjg.v26.i20.2570
Published online May 28, 2020. doi: 10.3748/wjg.v26.i20.2570
Serum outperforms plasma in small extracellular vesicle microRNA biomarker studies of adenocarcinoma of the esophagus
Karen Chiam, George C Mayne, Tingting Wang, David I Watson, Tanya S Irvine, Tim Bright, Lorelle T Smith, Sarah K Thompson, Damian J Hussey, Discipline of Surgery, College of Medicine and Public Health, Flinders University of South Australia, Adelaide, SA 5042, Australia
Karen Chiam, George C Mayne, Tingting Wang, David I Watson, Tanya S Irvine, Tim Bright, Lorelle T Smith, Damian J Hussey, Flinders Health and Medical Research Institute Cancer Program, College of Medicine and Public Health, Flinders University, Adelaide, SA 5042, Australia
Lorelle T Smith, Discipline of Surgery, The University of Adelaide, Adelaide, SA 5005, Australia
Imogen A Ball, Joanne M Bowen, Adelaide Medical School, The University of Adelaide, Adelaide, SA 5005, Australia
Dorothy M Keefe, Faculty of Health Sciences, The University of Adelaide, Adelaide, SA 5005, Australia
Author contributions: Hussey DJ conceived and supervised the study; Chiam K, Mayne GC, Watson DI, Bowen JM, Keefe DM, Thompson SK and Hussey DJ contributed to study design; Watson DI, Irvine TS, Bright T, Smith LT and White I collected patient blood samples; Wang T processed patient blood samples and performed the laboratory assays; Chiam K, Mayne GC and Hussey DJ analysed the data; Chiam K wrote the first draft of the paper; All authors contributed to revision of the manuscript in its final version.
Supported by National Health and Medical Research Council (NHMRC) Project Funding , No. APP1104281 ; and NHMRC Centres of Research Excellence (CRE) Grant , No. APP1040947 .
Institutional review board statement: Ethical approval was obtained from the Southern Adelaide Clinical Human Research Ethics Committee and the Royal Adelaide Hospital Research Committee.
Conflict-of-interest statement: The authors have no conflict of interest to declare.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Damian J Hussey, PhD, Senior Scientist, Discipline of Surgery, College of Medicine and Public Health, Flinders University of South Australia, Bedford Park, Adelaide, SA 5042, Australia. damian.hussey@flinders.edu.au
Received: January 1, 2020
Peer-review started: January 1, 2020
First decision: January 19, 2020
Revised: March 2, 2020
Accepted: May 13, 2020
Article in press: May 13, 2020
Published online: May 28, 2020
Processing time: 148 Days and 11 Hours
Peer-review started: January 1, 2020
First decision: January 19, 2020
Revised: March 2, 2020
Accepted: May 13, 2020
Article in press: May 13, 2020
Published online: May 28, 2020
Processing time: 148 Days and 11 Hours
Core Tip
Core tip: Current evidence suggests that circulating small extracellular vesicles (sEVs) function as delivery cargo shuttles for various molecules. MicroRNAs are small non-coding RNAs with important roles in the regulation of gene expression, are often dysregulated in diseases, and are relatively stable in the circulation. MicroRNAs circulating in sEVs are consequently considered as highly suitable candidates for use as non-invasive biomarkers. Extracellular vesicle preparations derived from serum and plasma are recognised to be enriched in sEVs, but not purely comprised of them. Most circulating sEV microRNA biomarker studies have used plasma, but here we show that sEVs isolated from serum are less contaminated with blood cell and protein-associated microRNAs.