Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 21, 2019; 25(39): 5961-5972
Published online Oct 21, 2019. doi: 10.3748/wjg.v25.i39.5961
Construction of a replication-competent hepatitis B virus vector carrying secreted luciferase transgene and establishment of new hepatitis B virus replication and expression cell lines
Jie Ruan, Cai-Yan Ping, Shuo Sun, Xin Cheng, Peng-Yu Han, Yin-Ge Zhang, Dian-Xing Sun
Jie Ruan, Cai-Yan Ping, Shuo Sun, Xin Cheng, Peng-Yu Han, Yin-Ge Zhang, Dian-Xing Sun, The Liver Disease Center of Chinese People’s Liberation Army, the 980th Hospital of Chinese People’s Liberation Army Joint Logistics Support Force, Shijiazhuang 050082, Hebei Province, China
Jie Ruan, Department of Infection and Liver Disease, Shannxi University of Chinese Medicine Affiliated Hospital, Xianyang 712000, Shannxi Province, China
Author contributions: Sun DX developed the methodology and provided funding; Ruan J, Sun S, Cheng X, and Sun DX designed and coordinated the research; Ruan J, Ping CY, Sun S, Han PY, and Zhang YG performed the majority of the experiments and analyzed the data; Ruan J and Sun DX wrote the manuscript; Ruan J, Ping CY, and Sun S contributed equally to this work.
Supported by the National Natural Science Foundation of China, No. 81672041; and the National Major Science and Technology Special Project for Infectious Diseases of China, No. 2012ZX10004503-012.
Institutional review board statement: Because no animals and patients were involved in this study, the IRB chose to waive this requirement.
Institutional animal care and use committee statement: Because no animals were involved in this study, the Institutional Animal Care and Use Committee chose to waive this requirement.
Conflict-of-interest statement: The authors declare no conflicts of interest.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and because no animals were involved in this study, the ARRIVE guidelines are not applicable.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Dian-Xing Sun, PhD, Doctor, Professor, The Liver Disease Center of Chinese People’s Liberation Army, the 980th Hospital of Chinese People’s Liberation Army Joint Logistics Support Force, Zhongshanxi Street, Shijiazhuang 050082, Hebei Province, China. sundianxing@hotmail.com
Telephone: +86-13081100156
Received: May 20, 2019
Peer-review started: May 20, 2019
First decision: July 21, 2019
Revised: August 8, 2019
Accepted: September 13, 2019
Article in press: September 13, 2019
Published online: October 21, 2019
Processing time: 154 Days and 22.7 Hours
Core Tip

Core tip: In this research, we constructed a replication-competent hepatitis B virus (HBV) vector carrying a secreted luciferase transgene and established HBV replication and expression cell lines that could stably secrete secretory Nanoluc Luciferase recombinant viral particles. This vector is a convenient and quantifiable tool for monitoring HBV replication, transcription, and expression. It is safe to assume that this new HBV replication system ought to be used in HBV molecular biology research, such as for discovering inhibitors that affect virus infection, entry, transcription, translation, reverse transcription, and replication.