Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 14, 2019; 25(34): 5134-5151
Published online Sep 14, 2019. doi: 10.3748/wjg.v25.i34.5134
Effect of Tong Xie Yao Fang on endogenous metabolites in urine of irritable bowel syndrome model rats
Xue-Ying Zhao, Jian-Wei Wang, Yue Yin, Kai Li, Miao Zhang, Fu-Ping Yan
Xue-Ying Zhao, Jian-Wei Wang, Yue Yin, Kai Li, Miao Zhang, Fu-Ping Yan, School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, China
Author contributions: Zhao XY and Wang JW performed most of the experiments, analyzed the experimental results, interpreted them and wrote the manuscript; Yin Y, Li K, Zhang M and Yan FP performed some experiments and interpretation of the results; Wang JW participated in the testing factor design and revised the manuscript. She is also the corresponding author; All co-authors participated in writing and checking the manuscript and approved the submitted manuscript.
Supported by the National Natural Science Foundation of China, No. 81573870; the Eighth Special Subsidy Project of China Post-Doctoral Science Foundation, No. 2015T80376; Postdoctoral Science-Research Developmental Foundation of China, No. 2013M531079; National Science Foundation of Heilongjiang Province, No. H2015020; Heilongjiang University of Traditional Chinese Medicine Outstanding Innovative Talents Support Project (Outstanding Young Academic Leaders), Postdoctoral Science-Research Developmental Foundation of Heilongjiang Province, No. LBH-Q12009; Youth Academic Backbone Fund of Heilongjiang Province Education Department, No. 1253G053; Youth Science and Technology Project of Traditional Chinese Medicine Department of Heilongjiang Province, No. ZQG-034.
Institutional review board statement: This study was approved by the Ethics Committee of the Heilongjiang University of Chinese Medicine.
Institutional animal care and use committee statement: This study was approved by the animal care and use committee of the Heilongjiang University of Chinese Medicine.
Conflict-of-interest statement: Authors declare no conflicts of interest.
Data sharing statement: The detailed experimental methods are available from the corresponding author at xueyingzhao2010@126.com and wangjianwei140918@126.com.
ARRIVE guidelines statement: The ARRIVE Guidelines have been adopted.
Open-Access: This is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Jian-Wei Wang, PhD, Professor, School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, Heilongjiang Province, China. wangjianwei140918@126.com
Telephone: +86-451-82193640
Received: April 18, 2019
Peer-review started: April 18, 2019
First decision: July 22, 2019
Revised: July 30, 2019
Accepted: August 19, 2019
Article in press: August 19, 2019
Published online: September 14, 2019
Processing time: 147 Days and 7.8 Hours
Core Tip

Core tip: Effects of Tong Xie Yao Fang on endogenous metabolites in urine of irritable bowel syndrome model rats were investigated through ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry. Tong Xie Yao Fang has the function of callback endogenous metabolite. Nine potential biomarkers were identified, and six main metabolic pathways were analyzed. They were related to neurotransmitter metabolism, inflammatory immunity, emotional changes and energy metabolism in irritable bowel syndrome disease, which may be the biological basis of irritable bowel syndrome spleen deficiency and liver hyperactivity syndrome.