Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 28, 2019; 25(32): 4629-4660
Published online Aug 28, 2019. doi: 10.3748/wjg.v25.i32.4629
Review of current diagnostic methods and advances in Helicobacter pylori diagnostics in the era of next generation sequencing
Daniel Pohl, Peter M Keller, Valentine Bordier, Karoline Wagner
Daniel Pohl, Valentine Bordier, Division of Gastroenterology, University Hospital of Zurich, Zurich 8006, Switzerland
Peter M Keller, Institute for Infectious Diseases, University of Bern, Bern 3010, Switzerland
Karoline Wagner, Institute of Medical Microbiology, University of Zurich, Zurich 8006, Switzerland
Author contributions: Pohl D, Keller PM, and Wagner K designed the study; Pohl D, Keller PM, Bordier V and Wagner K did the literature search and analysed the data; Pohl D, Keller PM, Bordier V and Wagner K wrote and edited the manuscript.
Conflict-of-interest statement: None of the authors have any potential conflicts of interest to declare.
Open-Access: This article is an open-access article which was selected byan in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Karoline Wagner, PhD, Postdoc, Division of Laboratory Medicine, University Hospital of Basel, Petersgraben 4, Basel 4031, Switzerland.
Telephone: +41-61-2653606 Fax: +41-61-3028004
Received: May 2, 2019
Peer-review started: May 4, 2019
First decision: May 24, 2019
Revised: June 25, 2019
Accepted: July 19, 2019
Article in press: July 19, 2019
Published online: August 28, 2019
Core Tip

Core tip: With worldwide increasing antibiotic resistance in Helicobacter pylori (H. pylori), drug resistance phenotypes should be determined prior to the administration of antibiotic eradication regimens. Our literature search yielded studies that focused on the prediction of drug resistance phenotypes in H. pylori based on the presence of certain point mutations in the bacterium’s genome using next generation sequencing (NGS) technology. Thus, NGS technology may enable the implementation of rapid and accurate genotypic drug susceptibility testing prior to the administration of antimicrobial therapy. This may increase H. pylori eradication rates and ultimately improve patient management.