Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 21, 2019; 25(23): 2911-2923
Published online Jun 21, 2019. doi: 10.3748/wjg.v25.i23.2911
Proteomics of the mediodorsal thalamic nucleus of rats with stress-induced gastric ulcer
Sheng-Nan Gong, Jian-Ping Zhu, Ying-Jie Ma, Dong-Qin Zhao
Sheng-Nan Gong, Jian-Ping Zhu, Ying-Jie Ma, Dong-Qin Zhao, College of Life Sciences, Shandong Normal University, Jinan 250014, Shandong Province, China
Author contributions: Zhao DQ and Zhu JP designed the experiments; Ma YJ and Gong SN performed the experiments; Zhao DQ performed mass spectrometry analysis and wrote the manuscript; Gong SN analyzed the protemoics data; Zhu JP analyzed the GM and EI data; Ma YJ analyzed the WB data; all the authors approved the final version of the manuscript for publication.
Supported by National Natural Science Foundation of China, No. 31501861; and Natural Science Foundation of Shandong Province, China, No. ZR2015CM013.
Institutional animal care and use committee statement: All procedures were approved by the Institutional Animal Care and Use Committee of Shandong Normal University (Jinan, People’s Republic of China; No. AEECSDNU2018003).
Conflict-of-interest statement: The authors have no conflicts of interest to declare.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Dong-Qin Zhao, PhD, Associate Professor, College of Life Sciences, Shandong Normal University, No. 88, East Wenhua Road, Lixia District, Jinan 250014, Shandong Province, China.
Telephone: +86-531-86180745
Received: January 27, 2019
Peer-review started: January 27, 2019
First decision: March 20, 2019
Revised: May 8, 2019
Accepted: May 18, 2019
Article in press: May 18, 2019
Published online: June 21, 2019
Core Tip

Core tip: Quantitative proteomic analysis was used to screen for differentially expressed proteins in the mediodorsal thalamic nucleus of rats subjected to restraint water-immersion stress. A total of 65 dysregulated proteins were identified, which are mainly involved in the neurological disease signalling pathways. Meanwhile, the 31 upregulated proteins are primarily related to cell division, whereas the 34 downregulated proteins are related to neuron morphogenesis and neurotransmitter regulation. Furthermore, glycogen synthase kinase-3 beta might be related to the central mechanism through which restraint water-immersion stress gives rise to stress-induced gastric ulcer (SGU). Proteomic analysis elucidated the nervous system molecular targets of SGU.