Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 7, 2019; 25(17): 2071-2085
Published online May 7, 2019. doi: 10.3748/wjg.v25.i17.2071
Herbs-partitioned moxibustion alleviates aberrant intestinal epithelial cell apoptosis by upregulating A20 expression in a mouse model of Crohn’s disease
Jing Zhou, Lu-Yi Wu, Liu Chen, Ya-Jing Guo, Yi Sun, Tao Li, Ji-Meng Zhao, Chun-Hui Bao, Huan-Gan Wu, Yin Shi
Jing Zhou, Liu Chen, Ya-Jing Guo, Yi Sun, Tao Li, Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Lu-Yi Wu, Qigong Institute, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China
Ji-Meng Zhao, Chun-Hui Bao, Huan-Gan Wu, Yin Shi, Key Laboratory of Acupuncture and Immunological Effects, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Ji-Meng Zhao, Chun-Hui Bao, Huan-Gan Wu, Key Laboratory of Acupuncture and Immunological Effects, Shanghai Institute of Acupuncture-Moxibustion and Meridian, Shanghai 200030, China
Yin Shi, Outpatient Department, Shanghai Institute of Acupuncture-Moxibustion and Meridian, Shanghai 200030, China
Author contributions: Zhou J, Shi Y, and Wu HG designed the research; Zhou J, Chen L, Zhao JM, Guo YJ, Sun Y, and Li T performed the experiments; Wu LY, Zhou J, and Bao CH collected and analyzed the data; Zhou J wrote the manuscript; all authors reviewed the manuscript prior to its submission, and read and approved the final manuscript.
Supported by National Natural Science Foundation of China, No. 81273844 and No. 81473757; National Key Basic Research Program of China (973 Program), No. 2015CB554500; and Shanghai Rising-Star Program, No. 16QA1403400.
Institutional animal care and use committee statement: All animal experiments in this study were performed under guidelines approved by the Animal Ethics Committee of the Shanghai University of Traditional Chinese Medicine (No. 2013025).
Conflict-of-interest statement: The authors declare no conflicts of interest.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The manuscript was prepared according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Yin Shi, PhD, Doctor, Professor, Outpatient Department, Shanghai Institute of Acupuncture-Moxibustion and Meridian, 650 South Wanping Road, Shanghai 200030, China. flysy0636@163.com
Telephone: +86-21-64383910 Fax: +86-21-64390339
Received: January 13, 2019
Peer-review started: January 14, 2019
First decision: February 13, 2019
Revised: March 13, 2019
Accepted: March 15, 2019
Article in press: March 16, 2019
Published online: May 7, 2019
Processing time: 114 Days and 11.1 Hours
Core Tip

Core tip: We report our results derived from mice with A20 deletion in intestinal epithelial cells by inducing Crohn’s disease. The Crohn’s disease model was induced with 2,4,6-trinitrobenzene sulfonic acid. This study demonstrates for the first time that herbs-partitioned moxibustion can upregulate the expression of A20, resulting in downregulation of tumor necrosis factor receptor (TNFR) 1, TNFR1-associated death domain, and receptor-interacting protein 1 to alleviate increased cell apoptosis in the intestinal epithelial barrier in Crohn's disease.