Basic Study
Copyright ©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 14, 2019; 25(10): 1210-1223
Published online Mar 14, 2019. doi: 10.3748/wjg.v25.i10.1210
Short hairpin RNA-mediated knockdown of nuclear factor erythroid 2-like 3 exhibits tumor-suppressing effects in hepatocellular carcinoma cells
Miao-Mei Yu, Yue-Hua Feng, Lu Zheng, Jun Zhang, Guang-Hua Luo
Miao-Mei Yu, Yue-Hua Feng, Lu Zheng, Jun Zhang, Guang-Hua Luo, Comprehensive Laboratory, the Third Affiliated Hospital of Soochow University, Changzhou 213003, Jiangsu Province, China
Author contributions: Yu MM and Luo GH designed this study; Feng YH and Zhang J performed the experiments; Yu MM, Feng YH, and Zheng L wrote the manuscript; Yu MM and Luo GH performed the bioinformatic prediction; Yu MM and Luo GH conducted statistical analysis; all authors read and approved the final manuscript.
Supported by the Changzhou High-Level Medical Talents Training Project, No. 2016ZCLJ002.
Institutional review board statement: The clinicopathological features involved in this study are all from the TCGA database, thus, no institutional review board document is provided.
Conflict-of-interest statement: None declared.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines and prepared the manuscript accordingly.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
Corresponding author: Guang-Hua Luo, MD, PhD, Professor, Senior Researcher, Comprehensive Laboratory, the Third Affiliated Hospital of Soochow University, No. 185, Juqian Street, Tianning District, Changzhou 213003, Jiangsu Province, China.
Telephone: +86-519-68870619 Fax: +86-519-86621235
Received: January 4, 2019
Peer-review started: January 4, 2019
First decision: January 23, 2019
Revised: February 13, 2019
Accepted: February 15, 2019
Article in press: February 16, 2019
Published online: March 14, 2019
Core Tip

Core tip: Nuclear factor erythroid 2-like 3 (NFE2L3), as a key regulator of the cellular stress response, is involved in the regulation of various tumors, whereas its role in hepatocellular carcinoma (HCC) has not been elucidated. Our present study identified that NFE2L3 was closely associated with the grade and stage of HCC patients based on The Cancer Genome Atlas database. Furthermore, our study showed that short hairpin RNA-mediated knockdown of NFE2L3 exhibited tumor-suppressing effects in HCC cells.