Basic Study
Copyright ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 21, 2018; 24(47): 5366-5378
Published online Dec 21, 2018. doi: 10.3748/wjg.v24.i47.5366
Bypassing major venous occlusion and duodenal lesions in rats, and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine
Fedor Amic, Domagoj Drmic, Zdenko Bilic, Ivan Krezic, Helena Zizek, Marina Peklic, Robert Klicek, Alen Pajtak, Enio Amic, Tinka Vidovic, Mislav Rakic, Marija Milkovic Perisa, Katarina Horvat Pavlov, Antonio Kokot, Ante Tvrdeic, Alenka Boban Blagaic, Mario Zovak, Sven Seiwerth, Predrag Sikiric
Fedor Amic, Domagoj Drmic, Zdenko Bilic, Ivan Krezic, Helena Zizek, Marina Peklic, Robert Klicek, Alen Pajtak, Enio Amic, Tinka Vidovic, Mislav Rakic, Marija Milkovic Perisa, Katarina Horvat Pavlov, Antonio Kokot, Ante Tvrdeic, Alenka Boban Blagaic, Mario Zovak, Sven Seiwerth, Predrag Sikiric, Department of Pharmacology, Medical Faculty, University of Zagreb, Zagreb 10000, Croatia
Fedor Amic, Domagoj Drmic, Zdenko Bilic, Ivan Krezic, Helena Zizek, Marina Peklic, Robert Klicek, Alen Pajtak, Enio Amic, Tinka Vidovic, Mislav Rakic, Marija Milkovic Perisa, Katarina Horvat Pavlov, Antonio Kokot, Ante Tvrdeic, Alenka Boban Blagaic, Mario Zovak, Sven Seiwerth, Predrag Sikiric, Department of Pathology, Medical Faculty, University of Zagreb, Zagreb 10000, Croatia
Author contributions: Amic F, Drmic D, Bilic Z, Krezic I, Zizek H, Peklic M, Klicek R, Pajtak A, Amic E, Vidovic T, Rakic M, Milkovic Perisa M, Horvat Pavlov K, Kokot A, Tvrdeic A and Boban Blagaic A performed experiments; Amic F, Drmic D, Bilic Z, Krezic I, Zizek H, Peklic M, Klicek R, Pajtak A, Amic E, Vidovic T, Rakic M, Milkovic Perisa M, Horvat Pavlov K, Kokot A, Tvrdeic A, Boban Blagaic A, Zovak M, Seiwerth S and Sikiric P analyzed data; Seiwerth S and Sikiric P contributed reagents and analytic tools; Amic F, Tvrdeic A, Seiwerth S and Sikiric P wrote the manuscript.
Institutional review board statement: The study was reviewed and approved by the Department of Veterinary, Ministry of Agriculture, Republic of Croatia, No: UP/I 322-01/07-01/210.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Uprava Za Veterinarstvo (NN 135/06).
Conflict-of-interest statement: The authors state that they have no conflicts of interest.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have checked the manuscript according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author to: Predrag Sikiric, MD, PhD, Professor, Department of Pharmacology, Medical Faculty, University of Zagreb, Salata 11, POB 916, Zagreb 10000, Croatia. sikiric@mef.hr
Telephone: +385-1-4566833 Fax: +385-1-4920050
Received: August 8, 2018
Peer-review started: August 9, 2018
First decision: October 5, 2018
Revised: November 26, 2018
Accepted: November 30, 2018
Article in press: November 30, 2018
Published online: December 21, 2018
Processing time: 136 Days and 15 Hours
Core Tip

Core tip: Up to now, application of prototype cytoprotective agent BPC 157 induced bypassing of occlusion, in rats underwent vessels occlusions, through rapid collaterals presentation, and lesions and whole consequent syndrome (ischemic colitis; deep vein thrombosis, Virchow triad) were attenuated. In rats underwent superior anterior pancreaticoduodenal vein-ligation, medication was with BPC 157, L-NAME; L-arginine, saline given alone and/or together at 1 min ligation time. Unlike severe course in controls, BPC 157 rats commonly exhibited strong attenuation of mucosal lesion and serosal congestion, since BPC 157 rescued original duodenal flow through inferior anterior pancreaticoduodenal vein to superior mesenteric vein flow.