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©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 14, 2018; 24(46): 5223-5233
Published online Dec 14, 2018. doi: 10.3748/wjg.v24.i46.5223
Published online Dec 14, 2018. doi: 10.3748/wjg.v24.i46.5223
Modulation of faecal metagenome in Crohn’s disease: Role of microRNAs as biomarkers
María Rojas-Feria, Teresa Romero-García, Marta Avilés-Recio, Manuel Castro-Fernandez, Lourdes Grande, José A Del Campo, Department of Digestive Diseases, Valme University Hospital, UGC Digestive Disease and CIBERehd, Servicio Andaluz de Salud, Seville E-41014, Spain
Jose Ángel Fernández Caballero-Rico, Natalia Chueca Porcuna, Federico García, Complejo Hospitalario Universitario de Granada, Microbiology, Granada E-18016, Spain
Jose Ángel Fernández Caballero-Rico, Facultad de ciencias de la salud. Universidad Europea Miguel de Cervantes, Madrid E-28280, Spain
Helena Pastor Ramírez, Manuel Romero-Gόmez, Institute of Biomedicine of Seville, Digestive Diseases, Hospital Universitario Virgen del Rocío and CIBERehd, Seville E-41013, Spain
Author contributions: Rojas-Feria M, Romero-García T, Romero-Gómez M, García F, Grande L and Del Campo JA; designed the study and wrote the manuscript. Fernández Caballero-Rico JÁ, Pastor Ramírez H and Avilés-Recio M; analyzed the data. Rojas-Feria M, Castro-Fernández M, Chueca Porcuna N, Grande L and Del Campo JA; contributed to perform experiments and data analysis
Supported by Instituto de Salud Carlos III , and Consejería de Salud Junta de Andalucía PI14/01349 .
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board of Valme Hospital of Seville University.
Conflict-of-interest statement: All authors declare there are no competing interests in this study.
Data sharing statement: All data regarding this manuscript will be available upon request.
ARRIVE guidelines statement: The manuscript was prepared according to the ARRIVE Guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author to: José A Del Campo, PhD, Senior Scientist, Department of Digestive Diseases, Valme University Hospital, UGC Digestive Disease and CIBERehd, Servicio Andaluz de Salud, Avda. Bellavista s/n, Sevilla E-41014, Spain. jantonio.delcampo@ciberehd.org
Telephone: +34-95-5015485
Received: September 19, 2018
Peer-review started: September 19, 2018
First decision: November 1, 2018
Revised: November 13, 2018
Accepted: November 16, 2018
Article in press: November 16, 2018
Published online: December 14, 2018
Processing time: 85 Days and 19.8 Hours
Peer-review started: September 19, 2018
First decision: November 1, 2018
Revised: November 13, 2018
Accepted: November 16, 2018
Article in press: November 16, 2018
Published online: December 14, 2018
Processing time: 85 Days and 19.8 Hours
Core Tip
Core tip: In this study, we have found a shift in microbial gut community composition that supports dysbiosis in Crohn’s disease (CD) patients. The greatest interest of our work is that we have only included new-onset adult CD patients. We found that active non-treated CD patients had a low Firmicutes/Bacteroidetes ratio, less biodiversity in the structure of microbial communities and a significantly different pattern on gut microbiota distribution. Three microRNAs (miRNAs) have been found induced in affected mucosa vs non-affected mucosa in CD, indicating that miRNA profile may serve as biomarker for active disease.