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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 21, 2017; 23(7): 1163-1170
Published online Feb 21, 2017. doi: 10.3748/wjg.v23.i7.1163
Published online Feb 21, 2017. doi: 10.3748/wjg.v23.i7.1163
Effect of biofilm formation by clinical isolates of Helicobacter pylori on the efflux-mediated resistance to commonly used antibiotics
Bahareh Attaran, Tahereh Falsafi, Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran 1993893973, Iran
Nassim Ghorbanmehr, Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran 1993893973, Iran
Author contributions: Attaran B and Falsafi T designed the study, wrote the manuscript, and provided vital analytical tools; Attaran B performed all experiments; Ghorbanmehr N participated in providing analytical tools and reading of the manuscript.
Institutional review board statement: The H. pylori strains used for this study were isolated from the patients who were admitted to Medical Centers for their persistent upper gastrointestinal problems, and for this reason they underwent endoscopy for biopsy. All routine biopsy specimens from the patients were taken after informed consent and the protocols, under which the biopsies for culture were obtained, were in accordance with the Helsinki Declaration of 1975.
Conflict-of-interest statement: The authors have no conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Tahereh Falsafi, Professor, Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Vanak Village Street, Tehran 1993893973, Iran. falsafi.tahereh@yahoo.com
Telephone: +98-21-88044040
Received: September 23, 2016
Peer-review started: September 25, 2016
First decision: October 20, 2016
Revised: November 11, 2016
Accepted: January 4, 2017
Article in press: January 4, 2017
Published online: February 21, 2017
Processing time: 149 Days and 21.1 Hours
Peer-review started: September 25, 2016
First decision: October 20, 2016
Revised: November 11, 2016
Accepted: January 4, 2017
Article in press: January 4, 2017
Published online: February 21, 2017
Processing time: 149 Days and 21.1 Hours
Core Tip
Core tip: The current study has addressed the co-incidence of biofilm formation by Helicobacter pylori (H. pylori) and decreased susceptibility to antibiotics. The results demonstrated a significantly higher expression of two efflux pump genes, hp1165 and hefA (involved in the specific resistance to tetracycline and multidrug resistance, respectively), in the biofilm-forming cells as compared to the planktonic cells. There was also association between H. pylori biofilm formation and decreased susceptibility to antibiotics. This event would probably be involved in the failure of H. pylori eradication and might be beneficial for developing new therapeutic approaches for this infection.