Basic Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 7, 2017; 23(33): 6077-6087
Published online Sep 7, 2017. doi: 10.3748/wjg.v23.i33.6077
High yield reproducible rat model recapitulating human Barrett’s carcinogenesis
Daisuke Matsui, Ashten N Omstead, Juliann E Kosovec, Yoshihiro Komatsu, Emily J Lloyd, Hailey Raphael, Ronan J Kelly, Ali H Zaidi, Blair A Jobe
Daisuke Matsui, Ashten N Omstead, Juliann E Kosovec, Yoshihiro Komatsu, Emily J Lloyd, Hailey Raphael, Ali H Zaidi, Blair A Jobe, Esophageal and Lung Institute, Allegheny Health Network, Pittsburgh, PA 15224, United States
Daisuke Matsui, Department of Gastroenterological Surgery, Kanazawa University Hospital, Kanazawa, Ishikawa 920-1192, Japan
Ronan J Kelly, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Hospital, Baltimore, MD 21231, United States
Author contributions: Matsui D, Omstead AN, and Kosovec JE performed the majority of the experiments, analyzed the data, and wrote the manuscript; Komatsu Y, Lloyd E and Raphael H were instrumental in the model development; Zaidi AH, Kelly RJ, and Jobe BA designed and coordinated the research and wrote the manuscript.
Institutional animal care and use committee statement: This study was conducted with approval from the Institutional Animal Care and Use Committee of Allegheny General Hospital in Pittsburgh, Pennsylvania under Protocol #992.
Conflict-of-interest statement: The authors have no conflicts to report.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Blair A Jobe, MD, Esophageal and Lung Institute, Allegheny Health Network, 4600 North Tower, 4800 Friendship Avenue, Pittsburgh, PA 15224, United States. blair.jobe@ahn.org
Telephone: +1-412-5784026 Fax: +1412-5781434
Received: May 10, 2017
Peer-review started: May 12, 2017
First decision: June 5, 2017
Revised: June 23, 2017
Accepted: July 24, 2017
Article in press: July 24, 2017
Published online: September 7, 2017
Processing time: 120 Days and 5.4 Hours
Core Tip

Core tip: The current study reportsrefined surgical techniques with improved tumor burdens for the modified Levrat model of end-to-side esophagojejunostomy in a rat for future in vivo studies of esophageal adenocarcinoma (EAC). For the first time, the model was established with significantly reduced mortality and morbidity and further validated through evaluation of conserved EAC disease progression markers, such as mucin and cytokeratins. The reported approach will allow for broader adoption of the model to allow for greater understanding of the complete disease progression spectrum from Barrett’s esophagus to metastatic EAC and aid in the development of novel therapeutics.