Prospective Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 7, 2016; 22(41): 9214-9221
Published online Nov 7, 2016. doi: 10.3748/wjg.v22.i41.9214
Molecular detection of Helicobacter pylori antibiotic resistance in stool vs biopsy samples
Denise E Brennan, Joseph Omorogbe, Mary Hussey, Donal Tighe, Grainne Holleran, Colm O’Morain, Sinéad M Smith, Deirdre McNamara
Denise E Brennan, Joseph Omorogbe, Mary Hussey, Donal Tighe, Grainne Holleran, Colm O’Morain, Sinéad M Smith, Deirdre McNamara, Department of Clinical Medicine, School of Medicine, Trinity College Dublin, 2 Dublin, Ireland
Sinéad M Smith, School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, 2 Dublin, Ireland
Author contributions: Smith SM and McNamara D contributed equally this work; Brennan DE, Omorogbe J, Hussey M, Tighe D, Holleran G, O’Morain C and McNamara D recruited patients and collected samples for the study; Brennan DE and Smith SM conducted experiments, analysed data and wrote the manuscript; all authors critically reviewed and approved the manuscript prior to publication.
Institutional review board statement: The study was reviewed and approved by the Adelaide and Meath Hospital Research Ethics Committee.
Conflict-of-interest statement: No potential conflicts of interest relevant to this article were reported.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Sinéad M Smith, PhD, Assistant Professor, Department of Clinical Medicine, School of Medicine, Trinity College Dublin, Tallaght, 2 Dublin, Ireland. smithsi@tcd.ie
Telephone: +35-318-961385 Fax: +35-318-962988
Received: August 9, 2016
Peer-review started: August 11, 2016
First decision: September 12, 2016
Revised: September 27, 2016
Accepted: October 19, 2016
Article in press: October 19, 2016
Published online: November 7, 2016
Processing time: 88 Days and 17 Hours
Core Tip

Core tip: The successful detection of clarithromycin and/or fluoroquinolone resistant Helicobacter pylori (H. pylori) infections by non-invasive methods would enable a widespread assessment of resistance rates. Here we evaluate the GenoType HelicoDR assay for the detection of clarithromycin and fluoroquinolone resistance using DNA isolated from stool samples compared to biopsy samples. Although results using this assay on biopsy tissue have previously been shown to correspond well with culture and antimicrobial susceptibility testing, there was weak correlation between results obtained using biopsy vs stool samples in our study. Further studies are required to optimise the non-invasive detection of clarithromycin and fluoroquinolone resistant H. pylori infection.